Home LiteratureArticle Details
PMID: 1961719 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phosphorylation-dependent regulation of axon fasciculation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10548-52

Cervello M, Lemmon V, Landreth G, Rutishauser U

Abstract

Axons often grow along other axons to produce bundles called fascicles, and a number of cell adhesion molecules (CAMs) found on axon surfaces contribute to this process. The surprising observation that Fab fragments against individual CAMs can completely block fascicle formation suggests that the different axon-associated CAMs are functionally linked. The present studies investigate whether such a linkage might reflect intracellular regulatory mechanisms. Results obtained with chicken retinal explants in culture indicate that fasciculation is highly sensitive to cytoplasmic protein phosphorylation by means of a mechanism that does not alter levels of CAM expression. Moreover, the potent effect of individual Fabs on fasciculation disappears with enhanced phosphorylation. These observations suggest that growing axons possess a general regulatory process for the multiple CAMs that participate in fasciculation.

MeSH Terms
Animals Axons/drug effects,physiology Cell Adhesion Molecules, Neuronal/physiology Chick Embryo Culture Techniques/methods Ethers, Cyclic/pharmacology Immunoglobulin Fab Fragments Neurites/drug effects,physiology Okadaic Acid Phosphorylation Retina/physiology Retinal Ganglion Cells/drug effects,physiology Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Cell Adhesion Molecules, Neuronal Ethers, Cyclic Immunoglobulin Fab Fragments Okadaic Acid Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cervello M
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Lemmon V
Landreth G
Rutishauser U
References (31)
31 references, click to expand
  1. Nerve growth factor.
    Physiol Rev. 1968 Jul;48(3):534-69 PMID: 4875350
  2. Adhesion among neural cells of the chick embryo. IV. Role of the cell surface molecule CAM in the formation of neurite bundles in cultures of spinal ganglia.
    J Cell Biol. 1978 Nov;79(2 Pt 1):382-93 PMID: 569154
  3. Effects of fasciculation on the outgrowth of neurites from spinal ganglia in culture.
    J Cell Biol. 1980 NOV;87(2 Pt 1):370-8 PMID: 7430247
  4. Oriented axon outgrowth from avian embryonic retinae in culture.
    Dev Biol. 1983 Jan;95(1):56-64 PMID: 6825932
  5. Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5762-6 PMID: 6577452
  6. Biochemical properties of the nerve growth factor-inducible large external (NILE) glycoprotein.
    J Neurosci. 1983 Dec;3(12):2420-30 PMID: 6655495
  7. Comparison of two cell surface molecules involved in neural cell adhesion.
    EMBO J. 1984 Feb;3(2):461-5 PMID: 6201360
  8. Involvement of the nerve growth factor-inducible large external glycoprotein (NILE) in neurite fasciculation in primary cultures of rat brain.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1276-80 PMID: 3856258
  9. Membrane glycoproteins involved in neurite fasciculation.
    J Cell Biol. 1987 Feb;104(2):343-53 PMID: 3805123
  10. Regulation of transmembrane signaling by receptor phosphorylation.
    Cell. 1987 Mar 27;48(6):913-22 PMID: 3030559
  11. Neurite outgrowth on muscle cell surfaces involves extracellular matrix receptors as well as Ca2+-dependent and -independent cell adhesion molecules.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2555-9 PMID: 3031688
  12. An L1-like molecule, the 8D9 antigen, is a potent substrate for neurite extension.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7753-7 PMID: 3478724
  13. Neurofascin: a novel chick cell-surface glycoprotein involved in neurite-neurite interactions.
    Cell. 1987 Dec 4;51(5):841-9 PMID: 3677174
  14. The neural cell adhesion molecule (NCAM) as a regulator of cell-cell interactions.
    Science. 1988 Apr 1;240(4848):53-7 PMID: 3281256
  15. Neurite outgrowth in individual neurons of a neuronal population is differentially regulated by calcium and cyclic AMP.
    J Neurosci. 1988 May;8(5):1704-11 PMID: 2835450
  16. Cell adhesion molecules in vertebrate neural development.
    Physiol Rev. 1988 Jul;68(3):819-57 PMID: 3293093
  17. N-cadherin, NCAM, and integrins promote retinal neurite outgrowth on astrocytes in vitro.
    J Cell Biol. 1988 Sep;107(3):1177-87 PMID: 3262111
  18. Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism.
    Nature. 1989 Jan 5;337(6202):78-81 PMID: 2562908
  19. Regulation of protein kinase C by nerve growth factor, epidermal growth factor, and phorbol esters in PC12 pheochromocytoma cells.
    J Biol Chem. 1989 May 25;264(15):8646-52 PMID: 2785993
  20. Identification of two protein kinases that phosphorylate the neural cell-adhesion molecule, N-CAM.
    J Neurosci. 1989 Jun;9(6):1883-96 PMID: 2542481
  21. The effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on axonal elongation and fasciculation.
    Anat Embryol (Berl). 1989;179(5):511-8 PMID: 2729610
  22. Neural cell recognition molecule F11: membrane interaction by covalently attached phosphatidylinositol.
    Biochem Biophys Res Commun. 1989 Jun 15;161(2):931-8 PMID: 2735929
  23. Protein-tyrosine phosphatases: the other side of the coin.
    Cell. 1989 Sep 22;58(6):1013-6 PMID: 2550140
  24. A protein kinase activity is associated with and specifically phosphorylates the neural cell adhesion molecule L1.
    J Neurochem. 1989 Nov;53(5):1471-8 PMID: 2677246
  25. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Nature. 1989 Oct 19;341(6243):619-24 PMID: 2477710
  26. Global modulation of the epidermal growth factor receptor is triggered by occupancy of only a few receptors. Evidence for a binary regulatory system in normal human fibroblasts.
    J Biol Chem. 1989 Nov 15;264(32):18912-20 PMID: 2808398
  27. A homologue of the axonally secreted protein axonin-1 is an integral membrane protein of nerve fiber tracts involved in neurite fasciculation.
    J Cell Biol. 1989 Nov;109(5):2363-78 PMID: 2509484
  28. A threshold effect of the major isoforms of NCAM on neurite outgrowth.
    Nature. 1990 Feb 1;343(6257):464-6 PMID: 2153939
  29. Neural cell adhesion molecules influence second messenger systems.
    Neuron. 1989 Jul;3(1):13-20 PMID: 2559759
  30. The role of cell adhesion molecules in neurite outgrowth on Müller cells.
    Dev Biol. 1990 Mar;138(1):82-93 PMID: 2407579
  31. Protein kinase C is involved in laminin stimulation of neurite outgrowth.
    Neuron. 1989 Sep;3(3):287-97 PMID: 2561968
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10548
Published
1991-12-01
Pages
10548-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52966
Subset
IM
Grants
NEI NIH HHS · R01 EY005285 · United States
NEI NIH HHS · EY05285 · United States
NEI NIH HHS · EY06107 · United States
NICHD NIH HHS · HD18369 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.23.10548 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2