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PMID: 1961744 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunological identification and characterization of a delayed rectifier K+ channel polypeptide in rat brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10764-8

Trimmer JS

Abstract

Antibodies specific for the drk1 polypeptide were used to characterize the corresponding protein in rat brain. Recombinant and synthetic immunogens containing fragments of the drk1 polypeptide were produced. Antibodies raised to these immunogens display monospecific reactions with the same 130-kDa polypeptide on immunoblots of adult rat brain membranes. Immunoprecipitation of 125I-labeled brain membranes identifies a 38-kDa peptide in tight association with the drk1 polypeptide. Immunohistochemical staining of sections of adult rat cortex shows that drk1 protein is restricted to neurons, where staining is present on dendrites and cell bodies but not on axons. These studies point to the value of such immunological reagents to the further characterization of the components of this delayed rectifier K+ channel in the mammalian central nervous system.

MeSH Terms
Animals Brain/cytology,physiology Cerebral Cortex/cytology,physiology Electrophoresis, Polyacrylamide Gel Immunoblotting Intracellular Membranes/physiology,ultrastructure Molecular Weight Neurons/cytology,physiology Polymerase Chain Reaction Potassium Channels/chemistry,genetics,immunology Protein Biosynthesis Rats Recombinant Fusion Proteins/analysis,immunology Synaptosomes/physiology,ultrastructure Transcription, Genetic
Chemicals
Potassium Channels Recombinant Fusion Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Trimmer J S
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215.
References (22)
22 references, click to expand
  1. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  2. Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells. Primary structure and functional expression from cDNAs.
    FEBS Lett. 1989 Dec 18;259(1):37-42 PMID: 2599109
  3. Determination of the subunit stoichiometry of a voltage-activated potassium channel.
    Nature. 1991 Mar 21;350(6315):232-5 PMID: 1706481
  4. Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7394-8 PMID: 2459705
  5. Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo.
    J Physiol. 1952 Apr;116(4):449-72 PMID: 14946713
  6. Heteromultimeric channels formed by rat brain potassium-channel proteins.
    Nature. 1990 Jun 7;345(6275):535-7 PMID: 2348860
  7. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
    Nature. 1989 Aug 24;340(6235):642-5 PMID: 2770868
  8. Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
    Nature. 1990 Jun 7;345(6275):530-4 PMID: 2112229
  9. Potassium channels expressed from rat brain cDNA have delayed rectifier properties.
    FEBS Lett. 1988 Dec 19;242(1):199-206 PMID: 2462513
  10. Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain.
    Neuron. 1990 Jun;4(6):929-39 PMID: 2361015
  11. An emerging pharmacology of peptide toxins targeted against potassium channels.
    J Membr Biol. 1988 Oct;105(2):95-111 PMID: 2464066
  12. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  13. Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4919-23 PMID: 2455300
  14. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  15. Immunological characterization of K+ channel components from the Shaker locus and differential distribution of splicing variants in Drosophila.
    Neuron. 1990 Jan;4(1):119-27 PMID: 2310570
  16. Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
    EMBO J. 1989 Nov;8(11):3235-44 PMID: 2555158
  17. A T3-like protein complex associated with the antigen receptor on murine T cells.
    Nature. 1986 Mar 20-26;320(6059):272-5 PMID: 2938011
  18. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
    J Biol Chem. 1981 Dec 10;256(23):11955-7 PMID: 7028745
  19. Starch-gel electrophoresis of anterior pituitary hormones.
    Nature. 1961 May 13;190:629-30 PMID: 13698890
  20. Expression of a cloned rat brain potassium channel in Xenopus oocytes.
    Science. 1989 Apr 14;244(4901):221-4 PMID: 2539643
  21. Immunological evidence for a relationship between the dendrotoxin-binding protein and the mammalian homologue of the Drosophila Shaker K+ channel.
    FEBS Lett. 1989 Jun 5;249(2):224-8 PMID: 2472291
  22. Assembly in vivo of mouse muscle acetylcholine receptor: identification of an alpha subunit species that may be an assembly intermediate.
    Cell. 1983 Oct;34(3):747-57 PMID: 6627392
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10764
Published
1991-12-01
Pages
10764-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53011
Subset
IM
Grants
NHLBI NIH HHS · HL 38156 · United States
NINDS NIH HHS · NS 17928 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.23.10764 PMC Full Text View journal details
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