Abstract
Anthrax toxin, a major virulence factor of Bacillus anthracis, gains entry into target cells by binding to either of 2 von Willebrand factor A domain-containing proteins, tumor endothelium marker-8 (TEM8) and capillary morphogenesis protein-2 (CMG2). The wide tissue expression of TEM8 and CMG2 suggest that both receptors could play a role in anthrax pathogenesis. To explore the roles of TEM8 and CMG2 in normal physiology, as well as in anthrax pathogenesis, we generated TEM8- and CMG2-null mice and TEM8/CMG2 double-null mice by deleting TEM8 and CMG2 transmembrane domains. TEM8 and CMG2 were found to be dispensable for mouse development and life, but both are essential in female reproduction in mice. We found that the lethality of anthrax toxin for mice is mostly mediated by CMG2 and that TEM8 plays only a minor role. This is likely because anthrax toxin has approximately 11-fold higher affinity for CMG2 than for TEM8. Finally, the CMG2-null mice are also shown to be highly resistant to B. anthracis spore infection, attesting to the importance of both anthrax toxin and CMG2 in anthrax infections.
MeSH Terms
Animals
Anthrax/metabolism,microbiology
Antigens, Bacterial/metabolism
Bacillus anthracis/metabolism,physiology
Bacterial Toxins/metabolism
Binding Sites
Biomarkers, Tumor
Blotting, Western
Cells, Cultured
Female
Fibroblasts/cytology,metabolism
Gene Expression
Genes, Essential/genetics
Host-Pathogen Interactions
Male
Membrane Proteins/genetics,metabolism
Mice
Mice, Knockout
Microfilament Proteins
Receptors, Cell Surface
Receptors, Peptide/genetics,metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spores, Bacterial/growth & development
Chemicals
Antigens, Bacterial
Antxr1 protein, mouse
Antxr2 protein, mouse
Bacterial Toxins
Biomarkers, Tumor
Membrane Proteins
Microfilament Proteins
Receptors, Cell Surface
Receptors, Peptide
anthrax toxin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Liu Shihui
Bacterial Toxins and Therapeutics Section, Laboratory of Bacterial Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
[email protected]
Crown Devorah
Miller-Randolph Sharmina
Moayeri Mahtab
Wang Hailun
Hu Haijing
Morley Thomas
Leppla Stephen H
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