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PMID: 19629172 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Pch2 links chromosome axis remodeling at future crossover sites and crossover distribution during yeast meiosis.

PLoS genetics ·Vol. 5 ·No. 7 ·2009-07-00 ·Pages e1000557

Joshi N, Barot A, Jamison C, Börner GV

Abstract

Segregation of homologous chromosomes during meiosis I depends on appropriately positioned crossovers/chiasmata. Crossover assurance ensures at least one crossover per homolog pair, while interference reduces double crossovers. Here, we have investigated the interplay between chromosome axis morphogenesis and non-random crossover placement. We demonstrate that chromosome axes are structurally modified at future crossover sites as indicated by correspondence between crossover designation marker Zip3 and domains enriched for axis ensemble Hop1/Red1. This association is first detected at the zygotene stage, persists until double Holliday junction resolution, and is controlled by the conserved AAA+ ATPase Pch2. Pch2 further mediates crossover interference, although it is dispensable for crossover formation at normal levels. Thus, interference appears to be superimposed on underlying mechanisms of crossover formation. When recombination-initiating DSBs are reduced, Pch2 is also required for viable spore formation, consistent with further functions in chiasma formation. pch2Delta mutant defects in crossover interference and spore viability at reduced DSB levels are oppositely modulated by temperature, suggesting contributions of two separable pathways to crossover control. Roles of Pch2 in controlling both chromosome axis morphogenesis and crossover placement suggest linkage between these processes. Pch2 is proposed to reorganize chromosome axes into a tiling array of long-range crossover control modules, resulting in chiasma formation at minimum levels and with maximum spacing.

MeSH Terms
Cell Cycle Proteins/metabolism Chromosomes, Fungal/metabolism Crossing Over, Genetic DNA-Binding Proteins/metabolism Gene Conversion Meiosis Nuclear Proteins/metabolism Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Ubiquitin-Protein Ligases/metabolism
Chemicals
Cell Cycle Proteins DMC1 protein, S cerevisiae DNA-Binding Proteins HOP1 protein, S cerevisiae Nuclear Proteins Pch2 protein, S cerevisiae RED1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases Zip3 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Joshi Neeraj
Department of Biological, Geological, and Environmental Sciences, Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, Ohio, United States of America.
Barot Aekam
Jamison Christine
Börner G Valentin
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1000557
Published
2009-07-00
Epub
2009-00-24
Pages
e1000557
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC2708914
Subset
IM
Grants
NIGMS NIH HHS · R15 GM080715 · United States
NIGMS NIH HHS · R15GM080715 · United States
Corrections
CommentIn
PMID: 19629169
External Links
PubMed source DOI: 10.1371/journal.pgen.1000557 PMC Full Text View journal details
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