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PMID: 1966012 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rat brain endoplasmic reticulum calcium pools are anatomically and functionally segregated.

Cell regulation ·Vol. 1 ·No. 10 ·1990-09-00 ·Pages 781-90

Verma A, Ross CA, Verma D, Supattapone S, Snyder SH

Abstract

Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate [I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated.

MeSH Terms
Animals Autoradiography Brain/anatomy & histology,metabolism Ca(2+) Mg(2+)-ATPase/genetics Calcium/metabolism Calcium Channels Calcium Radioisotopes Calcium-Transporting ATPases/genetics Endoplasmic Reticulum/drug effects,metabolism In Vitro Techniques Inositol 1,4,5-Trisphosphate/metabolism,pharmacology Inositol 1,4,5-Trisphosphate Receptors Male Oxalates/pharmacology RNA, Messenger/analysis,genetics Rats Rats, Inbred Strains Receptors, Cell Surface/metabolism Receptors, Cytoplasmic and Nuclear
Chemicals
Calcium Channels Calcium Radioisotopes Inositol 1,4,5-Trisphosphate Receptors Oxalates RNA, Messenger Receptors, Cell Surface Receptors, Cytoplasmic and Nuclear Inositol 1,4,5-Trisphosphate Ca(2+) Mg(2+)-ATPase Calcium-Transporting ATPases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verma A
Johns Hopkins University School of Public Health, Department of Toxicology, Baltimore, Maryland 21205.
Ross C A
Verma D
Supattapone S
Snyder S H
References (29)
29 references, click to expand
  1. Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons.
    Nature. 1989 Jun 8;339(6224):468-70 PMID: 2542801
  2. How do inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate regulate intracellular Ca2+?
    Biochem Soc Trans. 1989 Feb;17(1):6-9 PMID: 2535674
  3. GTP-activated communication between distinct inositol 1,4,5-trisphosphate-sensitive and -insensitive calcium pools.
    Nature. 1989 Jul 20;340(6230):236-9 PMID: 2787892
  4. Ethanol and inositol 1,4,5-trisphosphate release calcium from separate stores of brain microsomes.
    J Pharmacol Exp Ther. 1989 Sep;250(3):875-81 PMID: 2778717
  5. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  6. cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump.
    J Biol Chem. 1989 Nov 5;264(31):18561-8 PMID: 2553713
  7. Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400.
    Nature. 1989 Nov 2;342(6245):32-8 PMID: 2554142
  8. Distribution of two distinct Ca2+-ATPase-like proteins and their relationships to the agonist-sensitive calcium store in adrenal chromaffin cells.
    Nature. 1989 Nov 2;342(6245):72-4 PMID: 2530452
  9. Purified inositol 1,4,5-trisphosphate receptor mediates calcium flux in reconstituted lipid vesicles.
    Nature. 1989 Nov 2;342(6245):87-9 PMID: 2554143
  10. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.
    Biochem J. 1983 May 15;212(2):473-82 PMID: 6309146
  11. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
    J Histochem Cytochem. 1981 Apr;29(4):577-80 PMID: 6166661
  12. Calcium-sequestering cell organelles: in situ localization, morphological and functional characterization.
    Prog Histochem Cytochem. 1989;20(2):1-47 PMID: 2690182
  13. Functional importance of the synaptic plasma membrane calcium pump and sodium-calcium exchanger.
    J Biol Chem. 1984 Sep 10;259(17):10807-13 PMID: 6147347
  14. Specificity of inositol trisphosphate-induced calcium release from permeabilized Swiss-mouse 3T3 cells.
    Biochem J. 1984 Aug 15;222(1):269-72 PMID: 6332622
  15. Ca-pumps in smooth muscle: one in plasma membrane and another in endoplasmic reticulum.
    Cell Calcium. 1985 Jun;6(3):227-36 PMID: 2410130
  16. Guanosine 5'-triphosphate releases calcium from rat liver and guinea pig parotid gland endoplasmic reticulum independently of inositol 1,4,5-trisphosphate.
    FEBS Lett. 1986 Jul 7;202(2):267-73 PMID: 3487467
  17. myo-Inositol 1,4,5-trisphosphate mobilizes Ca2+ from isolated adipocyte endoplasmic reticulum but not from plasma membranes.
    Biochem J. 1986 May 15;236(1):37-44 PMID: 2947569
  18. Vasopressin and oxytocin mRNAs in adrenalectomized and Brattleboro rats: analysis by quantitative in situ hybridization histochemistry.
    Brain Res. 1986 Dec;387(3):231-41 PMID: 3828759
  19. Inositol 1,4,5-trisphosphate and 5'-GTP induce calcium release from different intracellular pools.
    FEBS Lett. 1987 Jun 22;218(1):153-8 PMID: 3496244
  20. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  21. Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.
    J Biol Chem. 1987 Sep 5;262(25):12132-6 PMID: 3040730
  22. Solubilization, purification, and characterization of an inositol trisphosphate receptor.
    J Biol Chem. 1988 Jan 25;263(3):1530-4 PMID: 2826483
  23. Heparin inhibits the inositol 1,4,5-trisphosphate-induced Ca2+ release from rat liver microsomes.
    FEBS Lett. 1988 Feb 8;228(1):57-9 PMID: 3257739
  24. Persistent inhibition by inositol 1,4,5-trisphosphate of oxalate-dependent 45calcium accumulation in permeable guinea-pig hepatocytes.
    Cell Calcium. 1988 Feb;9(1):9-16 PMID: 3258794
  25. Competitive, reversible, and potent antagonism of inositol 1,4,5-trisphosphate-activated calcium release by heparin.
    J Biol Chem. 1988 Aug 15;263(23):11075-9 PMID: 3136153
  26. A novel Ca2+ pump expressed in brain, kidney, and stomach is encoded by an alternative transcript of the slow-twitch muscle sarcoplasmic reticulum Ca-ATPase gene. Identification of cDNAs encoding Ca2+ and other cation-transporting ATPases using an oligonucleotide probe derived from the ATP-binding site.
    J Biol Chem. 1988 Oct 15;263(29):15032-40 PMID: 2844797
  27. Characterization of inositol 1,4,5-trisphosphate-stimulated calcium release from rat cerebellar microsomal fractions. Comparison with [3H]inositol 1,4,5-trisphosphate binding.
    Biochem J. 1988 Oct 15;255(2):677-83 PMID: 3264497
  28. Inositol 1,4,5-trisphosphate receptor binding: autoradiographic localization in rat brain.
    J Neurosci. 1989 Jan;9(1):339-46 PMID: 2536419
  29. Molecular recognition of inositol polyphosphates by intracellular receptors and metabolic enzymes.
    Trends Pharmacol Sci. 1989 Apr;10(4):139-44 PMID: 2546303
Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.1.10.781
Published
1990-09-00
Pages
781-90
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361662
Subset
IM
Grants
NIDA NIH HHS · DA-00074 · United States
NIGMS NIH HHS · GM-07309 · United States
NIMH NIH HHS · MH-18501 · United States
External Links
PubMed source DOI: 10.1091/mbc.1.10.781 PMC Full Text
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