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PMID: 1967600 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

F1C fimbriae of a uropathogenic Escherichia coli strain: genetic and functional organization of the foc gene cluster and identification of minor subunits.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 1114-20

Riegman N, Kusters R, Van Veggel H, Bergmans H, Van Bergen en Henegouwen P, Hacker J, Van Die I

Abstract

The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein which shows similarities to the subunit protein FocA. Apart from the FocA major subunits, purified F1C fimbriae contain at least two minor subunits, FocG and FocH. Minor proteins of similar size were observed in purified S fimbriae. Remarkably, some mutations in the foc gene cluster result in an altered fimbrial morphology, i.e., rigid stubs or long, curly fimbriae.

MeSH Terms

Bacterial Proteins/genetics Blotting, Western Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics,pathogenicity,ultrastructure Fimbriae, Bacterial/physiology Genes, Bacterial Humans Immunohistochemistry Microscopy, Electron Multigene Family Plasmids Restriction Mapping Urinary Tract Infections/microbiology

Chemicals

Bacterial Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Riegman N

Department of Molecular Cell Biology, University of Utrecht, The Netherlands.

Kusters R

Van Veggel H

Bergmans H

Van Bergen en Henegouwen P

Hacker J

Van Die I

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.172.2.1114-1120.1990

Published

1990-02-00

Pages

1114-20

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC208544

Subset

External Links

PubMed source DOI: 10.1128/jb.172.2.1114-1120.1990 PMC Full Text View journal details

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F1C fimbriae of a uropathogenic Escherichia coli strain: genetic and functional organization of the foc gene cluster and identification of minor subunits.

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