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PMID: 1968465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway.

The Journal of cell biology ·Vol. 110 ·No. 3 ·1990-03-00 ·Pages 637-49

Rothberg KG, Ying YS, Kolhouse JF, Kamen BA, Anderson RG

Abstract

The folate receptor, also known as the membrane folate-binding protein, is maximally expressed on the surface of folate-depleted tissue culture cells and mediates the high affinity accumulation of 5-methyltetrahydrofolic acid in the cytoplasm of these cells. Recent evidence suggests that this receptor recycles during folate internalization and that it is anchored in the membrane by a glycosyl-phosphatidylinositol linkage. Using quantitative immunocytochemistry, we now show that (a) this receptor is highly clustered on the cell surface; (b) these clusters are preferentially associated with uncoated membrane invaginations rather than clathrin-coated pits; and (c) the receptor is not present in endosomes or lysosomes. This receptor appears to physically move in and out of the cell using a novel uncoated pit pathway that does not merge with the clathrin-coated pit endocytic machinery.

MeSH Terms
Animals Carrier Proteins/metabolism,ultrastructure Cell Line Coated Pits, Cell-Membrane/metabolism,ultrastructure Endocytosis Endosomes/metabolism Fluorescent Antibody Technique Folate Receptors, GPI-Anchored Folic Acid/metabolism Freeze Etching Glycolipids/metabolism Glycosylphosphatidylinositols Humans KB Cells Microscopy, Electron Phosphatidylinositols/metabolism Receptors, Cell Surface/metabolism
Chemicals
Carrier Proteins Folate Receptors, GPI-Anchored Glycolipids Glycosylphosphatidylinositols Phosphatidylinositols Receptors, Cell Surface Folic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rothberg K G
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235.
Ying Y S
Kolhouse J F
Kamen B A
Anderson R G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.3.637
Published
1990-03-00
Pages
637-49
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116044
Subset
IM
Grants
NHLBI NIH HHS · HL20948 · United States
External Links
PubMed source DOI: 10.1083/jcb.110.3.637 PMC Full Text View journal details
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