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PMID: 19688273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro.

Molecular biology reports ·Vol. 37 ·No. 5 ·2010-06-00 ·Pages 2285-93

Hong L, Sun H, Lv X, Yang D, Zhang J, Shi Y

Abstract

Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 + or - 5.33 pg/ml) compared to the normal healthy volunteers (215.66 + or - 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.

MeSH Terms
Aged Biomarkers, Tumor Carcinoma, Non-Small-Cell Lung/blood,genetics,pathology Case-Control Studies Cell Adhesion Molecules/blood Cell Line, Tumor Cell Movement Cell Proliferation DNA, Recombinant/genetics Enzyme-Linked Immunosorbent Assay Epithelium/metabolism,pathology Female Gene Expression Regulation, Neoplastic Humans Lung Neoplasms/blood,genetics,pathology Male Mesoderm/metabolism,pathology Microscopy, Fluorescence Middle Aged Plasmids/genetics RNA, Messenger/genetics,metabolism Transfection Wound Healing
Chemicals
Biomarkers, Tumor Cell Adhesion Molecules DNA, Recombinant POSTN protein, human RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hong Lingzhi
Department of Respiratory Diseases, Jinling Hospital, School of Medicine, Nanjing University, 210002, Nanjing, Jiangsu Province, People's Republic of China.
Sun Huiming
Lv Xiaojing
Yang Di
Zhang Jinan
Shi Yi
References (39)
39 references, click to expand
  1. Differential expression of osteoblast-specific factor 2 and polymeric immunoglobulin receptor genes in nasopharyngeal carcinoma.
    Head Neck. 2005 Oct;27(10):873-82 PMID: 16136586
  2. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility.
    Cancer Res. 2002 Sep 15;62(18):5358-64 PMID: 12235007
  3. Cell adhesion and signalling by cadherins and Ig-CAMs in cancer.
    Nat Rev Cancer. 2004 Feb;4(2):118-32 PMID: 14964308
  4. Distinct mechanisms of tumor invasion and metastasis.
    Trends Mol Med. 2007 Dec;13(12):535-41 PMID: 17981506
  5. Prognostic significance of epithelial-mesenchymal and mesenchymal-epithelial transition protein expression in non-small cell lung cancer.
    Clin Cancer Res. 2008 Nov 15;14(22):7430-7 PMID: 19010860
  6. Complex networks orchestrate epithelial-mesenchymal transitions.
    Nat Rev Mol Cell Biol. 2006 Feb;7(2):131-42 PMID: 16493418
  7. Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the beta4 integrin and the PI3k pathway.
    Oncogene. 2007 Mar 29;26(14):2082-94 PMID: 17043657
  8. Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas.
    Cancer. 2001 Aug 15;92(4):843-8 PMID: 11550156
  9. E-Cadherin-dependent growth suppression is mediated by the cyclin-dependent kinase inhibitor p27(KIP1).
    J Cell Biol. 1998 Jul 27;142(2):557-71 PMID: 9679152
  10. Epithelial-mesenchymal transitions: twist in development and metastasis.
    Cell. 2004 Aug 6;118(3):277-9 PMID: 15294153
  11. Periostin induction in tumor cell line explants and inhibition of in vitro cell growth by anti-periostin antibodies.
    Carcinogenesis. 2005 May;26(5):908-15 PMID: 15731169
  12. Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymal interaction.
    Dev Dyn. 2004 Apr;229(4):857-68 PMID: 15042709
  13. E-cadherin engagement stimulates proliferation via Rac1.
    J Cell Biol. 2006 May 8;173(3):431-41 PMID: 16682529
  14. Evidence that fibroblasts derive from epithelium during tissue fibrosis.
    J Clin Invest. 2002 Aug;110(3):341-50 PMID: 12163453
  15. Patterns of gene expression associated with BMP-2-induced osteoblast and adipocyte differentiation of mesenchymal progenitor cell 3T3-F442A.
    J Bone Miner Metab. 2000;18(3):132-9 PMID: 10783846
  16. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells.
    J Cell Biol. 1982 Oct;95(1):333-9 PMID: 7142291
  17. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression.
    Mol Cell Biol. 2004 May;24(9):3992-4003 PMID: 15082792
  18. Cadherin-mediated adhesion is required for normal growth regulation of human gingival epithelial cells.
    Cell Adhes Commun. 1996 Jul;4(1):13-24 PMID: 8870970
  19. Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line.
    J Cell Biol. 1994 Oct;127(1):247-56 PMID: 7929567
  20. Genetic manipulation of periostin expression in the heart does not affect myocyte content, cell cycle activity, or cardiac repair.
    Circ Res. 2009 Jan 2;104(1):e1-7 PMID: 19038863
  21. The E-cadherin cell-cell adhesion complex and lung cancer invasion, metastasis, and prognosis.
    Lung Cancer. 2002 May;36(2):115-24 PMID: 11955645
  22. Elevated serum periostin levels in patients with bone metastases from breast but not lung cancer.
    Breast Cancer Res Treat. 2003 Feb;77(3):245-52 PMID: 12602924
  23. Structural basis of cell-cell adhesion by cadherins.
    Nature. 1995 Mar 23;374(6520):327-37 PMID: 7885471
  24. Regulation of cadherin adhesive activity.
    J Cell Biol. 2000 Feb 7;148(3):399-404 PMID: 10662767
  25. Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer.
    Cancer Res. 2006 Jul 15;66(14):6928-35 PMID: 16849536
  26. Beta-catenin and Tcfs in mammary development and cancer.
    J Mammary Gland Biol Neoplasia. 2003 Apr;8(2):145-58 PMID: 14635791
  27. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.
    Biochem J. 1993 Aug 15;294 ( Pt 1):271-8 PMID: 8363580
  28. Upregulated expression of periostin by hypoxia in non-small-cell lung cancer cells promotes cell survival via the Akt/PKB pathway.
    Cancer Lett. 2009 Aug 28;281(2):213-9 PMID: 19328625
  29. CAM-FGF receptor interactions: a model for axonal growth.
    Mol Cell Neurosci. 1996;8(2-3):99-111 PMID: 8918827
  30. Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway.
    Cancer Cell. 2004 Apr;5(4):329-39 PMID: 15093540
  31. Global cancer statistics, 2002.
    CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108 PMID: 15761078
  32. Altered expression of adhesion molecules and epithelial-mesenchymal transition in silica-induced rat lung carcinogenesis.
    Lab Invest. 2004 Aug;84(8):999-1012 PMID: 15195114
  33. Epithelial versus mesenchymal phenotype determines in vitro sensitivity and predicts clinical activity of erlotinib in lung cancer patients.
    Clin Cancer Res. 2005 Dec 15;11(24 Pt 1):8686-98 PMID: 16361555
  34. Periostin: novel diagnostic and therapeutic target for cancer.
    Histol Histopathol. 2007 Oct;22(10):1167-74 PMID: 17616943
  35. Quantitative evaluation of vimentin expression in tumour stroma of colorectal cancer.
    Br J Cancer. 2007 Mar 26;96(6):986-92 PMID: 17325702
  36. High-throughput tissue microarray analysis to evaluate genes uncovered by cDNA microarray screening in renal cell carcinoma.
    Am J Pathol. 1999 Apr;154(4):981-6 PMID: 10233835
  37. Expression of the periostin mRNA level in neuroblastoma.
    J Pediatr Surg. 2002 Sep;37(9):1293-7 PMID: 12194119
  38. Gaseous nitrogen oxide promotes human lung cancer cell line A549 migration, invasion, and metastasis via iNOS-mediated MMP-2 production.
    Toxicol Sci. 2008 Dec;106(2):364-75 PMID: 18796497
  39. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta.
    J Bone Miner Res. 1999 Jul;14(7):1239-49 PMID: 10404027
Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
1573-4978
DOI
10.1007/s11033-009-9721-1
Published
2010-06-00
Epub
2009-00-18
Pages
2285-93
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/s11033-009-9721-1 View journal details
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