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PMID: 1970238 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.

The Biochemical journal ·Vol. 266 ·No. 3 ·1990-03-15 ·Pages 917-9

Shackleton DR, Hulmes DJ

Abstract

A method is described for the purification of piglet skin lysyl oxidase from a crude urea extract by two simple chromatographic procedures. The method relies on the selective interaction between Sephacryl S-200 and lysyl oxidase that occurs in the absence of urea and in conditions of low ionic strength.

MeSH Terms
Acrylic Resins Amino Acid Oxidoreductases/isolation & purification Animals Chromatography, Affinity Protein-Lysine 6-Oxidase/isolation & purification Skin/enzymology Swine
Chemicals
Acrylic Resins sephacryl S 200 Amino Acid Oxidoreductases Protein-Lysine 6-Oxidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shackleton D R
Department of Biochemistry, University of Edinburgh Medical School, Scotland, U.K.
Hulmes D J
References (20)
20 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1990-03-15
Pages
917-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1131226
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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