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PMID: 19703400 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis.

Cell ·Vol. 138 ·No. 4 ·2009-08-21 ·Pages 750-9

Wu G, Park MY, Conway SR, Wang JW, Weigel D, Poethig RS

Abstract

The transition from the juvenile to the adult phase of shoot development in plants is accompanied by changes in vegetative morphology and an increase in reproductive potential. Here, we describe the regulatory mechanism of this transition. We show that miR156 is necessary and sufficient for the expression of the juvenile phase, and regulates the timing of the juvenile-to-adult transition by coordinating the expression of several pathways that control different aspects of this process. miR156 acts by repressing the expression of functionally distinct SPL transcription factors. miR172 acts downstream of miR156 to promote adult epidermal identity. miR156 regulates the expression of miR172 via SPL9 which, redundantly with SPL10, directly promotes the transcription of miR172b. Thus, like the larval-to-adult transition in Caenorhabditis elegans, the juvenile-to-adult transition in Arabidopsis is mediated by sequentially operating miRNAs. miR156 and miR172 are positively regulated by the transcription factors they target, suggesting that negative feedback loops contribute to the stability of the juvenile and adult phases.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant MicroRNAs/genetics,metabolism Trans-Activators Transcription Factors/metabolism
Chemicals
Arabidopsis Proteins MicroRNAs Mirn172 microRNA, Arabidopsis SPL10 protein, Arabidopsis SPL9 protein, Arabidopsis Trans-Activators Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wu Gang
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Park Mee Yeon
Conway Susan R
Wang Jia-Wei
Weigel Detlef
Poethig R Scott
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2009.06.031
Published
2009-08-21
Pages
750-9
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2732587
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051893-11 · United States
NIGMS NIH HHS · R56 GM051893 · United States
NIGMS NIH HHS · R56 GM051893-12 · United States
NIGMS NIH HHS · R01 GM051893-13 · United States
NIGMS NIH HHS · R01 GM051893 · United States
Corrections
CommentIn
PMID: 19703391
External Links
PubMed source DOI: 10.1016/j.cell.2009.06.031 PMC Full Text View journal details
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