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PMID: 197274 Published · ppublish English Journal Article

Analysis of polyoma virus DNA replicative intermediates by agarose gel electrophoresis.

Journal of virology ·Vol. 23 ·No. 3 ·1977-09-00 ·Pages 827-32

Martin RF

Abstract

Agarose gel electrophoresis has been used to fractionate polyoma virus DNA replicative intermediates (RI) according to maturity. Approximate electrophoretic mobility versus maturity relationships were obtained for both intact (supercoiled) and nicked (relaxed) RI. There was considerable overlap between the supercoiled and relaxed RI populations after electrophoretic fractionation. Intact RI could be recovered from preparative agarose gels for further analysis by centrifugation, electron microscopy, re-electrophoresis, or nuclease digestion.

MeSH Terms
DNA Replication DNA Restriction Enzymes/metabolism DNA, Viral/analysis,biosynthesis Deoxyribonucleases/metabolism Electrophoresis Electrophoresis, Agar Gel Polyomavirus/analysis,metabolism
Chemicals
DNA, Viral Deoxyribonucleases DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Martin R F
References (19)
19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.3.827-832.1977
Published
1977-09-00
Pages
827-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515896
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.3.827-832.1977 PMC Full Text View journal details
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