Isolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression.

Molecular & general genetics : MGG ·Vol. 154 ·No. 2 ·1977-07-20 ·Pages 213-20

Ciriacy M

Abstract

Yeast mutants deficient in the constitutive ADHI (adc 1) were used for the isolation of mutants with deficiencies of the intermediary carbon metabolism, and of mutants defective in carbon catabolite derepression. Mutants were recognized by their inability to grow on YEP-glycerol and/or on ethanol synthetic complete medium. They were either defective in isocitrate lyase (ic11), succinate dehydrogenase (sdh1), or malate dehydrogenase (mdh1, mdh2), mdh-mutants could not uniformely be appointed to one of the known MDH isozymes. Homozygous mdh and sdh1 diploids are unable to sporulate. Three gene loci could be identified by mutants pleiotropically defective in many or all of the enzymes tested In ccr 1 mutants, derepression of isocitrate lyase, fructose-1,6-diphosphatase, ADHII and possibly of the cytoplasmic MDH is prevented, whereas the mitochondrial TCA-cycle enzymes, succinate dehydrogenase and malate dehydrogenase, are not significantly affected. CCR2 and CCR3 have quite similar action spectra. Both genes are obviously necessary for derepression of all enzymes tested. It could be shown that ccr1, ccr2 and ccr3 mutants are not respiratory deficient.

MeSH Terms

Alcohol Oxidoreductases/metabolism Carbon/metabolism Enzyme Repression/drug effects Fructose-Bisphosphatase/metabolism Genes Glucose/pharmacology Isocitrate Lyase/metabolism Isoenzymes/metabolism Malate Dehydrogenase/metabolism Malate Synthase/metabolism Mutation Saccharomyces cerevisiae/metabolism Succinate Dehydrogenase/metabolism

Chemicals

Isoenzymes Carbon Alcohol Oxidoreductases Malate Dehydrogenase Succinate Dehydrogenase Malate Synthase Fructose-Bisphosphatase Isocitrate Lyase Glucose

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ciriacy M

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24 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330840

Published

1977-07-20

Pages

213-20

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330840

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Isolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression.

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