Home LiteratureArticle Details
PMID: 197490 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Strandedness of newly synthesized short pieces of polyoma DNA from isolated nuclei.

Nucleic acids research ·Vol. 4 ·No. 5 ·1977-00-00 ·Pages 1449-64

Flory PJ

Abstract

The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4- to 2.4-fold) of short pieces of the strand growing in the 3' leads to 5' direction was found.

MeSH Terms
Cell Line Cell Nucleus/metabolism DNA Replication DNA Restriction Enzymes DNA, Circular/biosynthesis,isolation & purification DNA, Single-Stranded/biosynthesis,isolation & purification DNA, Viral/biosynthesis,isolation & purification Molecular Weight Nucleic Acid Hybridization Nucleic Acid Renaturation Polyomavirus/metabolism
Chemicals
DNA, Circular DNA, Single-Stranded DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Flory P J
References (12)
12 references, click to expand
  1. The replication of polyoma DNA.
    J Gen Virol. 1973 Dec;21(3):515-21 PMID: 4128636
  2. Replication of polyoma DNA in isolated nuclei. 3. The nucleotide sequence at the RNA-DNA junction of nascent strands.
    J Mol Biol. 1974 Mar 25;84(1):197-216 PMID: 4364587
  3. Discontinuous elongation of both strands at the replication forks in polyoma DNA replication.
    Nat New Biol. 1973 Oct 17;245(146):203-5 PMID: 4355746
  4. Polyoma DNA: a physical map.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2077-81 PMID: 4365585
  5. Initiator RNA in discontinuous polyoma DNA synthesis.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4901-5 PMID: 4373733
  6. RNA-linked short DNA fragments during polyoma replication.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):412-5 PMID: 4346889
  7. Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.
    J Mol Biol. 1972 Dec 30;72(3):523-37 PMID: 4349757
  8. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  9. Orientation of the complementary strands of polyoma virus DNA with respect to the DNA physical map.
    J Virol. 1975 Jan;17(1):212-8 PMID: 173885
  10. In vitro polyoma DNA synthesis: self-annealing properties of short DNA chains.
    Cell. 1975 Jun;5(2):205-11 PMID: 166760
  11. A continuous electroelution method for the recovery of DNA restriction enzyme fragments.
    Anal Biochem. 1974 Aug;60(2):640-4 PMID: 4844577
  12. The origin of nascent single-stranded fragments in replicating TM DNA.
    J Mol Biol. 1975 Nov 15;98(4):719-37 PMID: 1104877
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.5.1449
Published
1977-00-00
Pages
1449-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343767
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.5.1449 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2