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PMID: 19765264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Live cell multicolor imaging of lipid droplets with a new dye, LD540.

Traffic (Copenhagen, Denmark) ·Vol. 10 ·No. 11 ·2009-11-00 ·Pages 1579-84

Spandl J, White DJ, Peychl J, Thiele C

Abstract

A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.

MeSH Terms

3T3-L1 Cells Adipocytes/chemistry Animals COS Cells Carcinoma, Hepatocellular/pathology Carrier Proteins/metabolism Cell Line, Tumor Chlorocebus aethiops Color Fluorescent Dyes/chemical synthesis,chemistry,metabolism Green Fluorescent Proteins/metabolism Humans Lipids/analysis Liver Neoplasms/pathology Membrane Proteins Mice Microscopy, Confocal Microtubules/metabolism Sensitivity and Specificity Spectrometry, Fluorescence Transfection Tubulin/metabolism

Chemicals

AUP1 protein, human Carrier Proteins Fluorescent Dyes Lipids Membrane Proteins Tubulin enhanced green fluorescent protein Green Fluorescent Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Spandl Johanna

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.

White Daniel J

Peychl Jan

Thiele Christoph

Article Info

Journal

Traffic (Copenhagen, Denmark)

Abbr.

Traffic

ISSN

1600-0854

DOI

10.1111/j.1600-0854.2009.00980.x

Published

2009-11-00

Epub

2009-00-02

Pages

1579-84

Language

English

Region

England

NLM ID

100939340

Subset

External Links

PubMed source DOI: 10.1111/j.1600-0854.2009.00980.x View journal details

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Live cell multicolor imaging of lipid droplets with a new dye, LD540.

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