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PMID: 1977765 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.

Journal of clinical microbiology ·Vol. 28 ·No. 9 ·1990-09-00 ·Pages 2051-8

Hermans PW, van Soolingen D, Dale JW, Schuitema AR, McAdam RA, Catty D, van Embden JD

Abstract

IS986 of Mycobacterium tuberculosis belongs to the IS3-like family of insertion sequences, and it has previously been shown to be present in multiple copies in the chromosome of M. tuberculosis. In this study we investigated the value of a IS986-based DNA probe in the diagnosis and epidemiology of tuberculosis. IS986 was found only in species belonging to the M. tuberculosis complex. Independent isolates of M. tuberculosis complex strains showed a very high degree of polymorphism of restriction fragments which contained IS986 DNA. In contrast, Mycobacterium bovis BCG vaccine strains as well as clinical isolates of M. bovis BCG contained one copy of IS986, which was present at the same location in the chromosome. Different M. tuberculosis isolates from a recent M. tuberculosis outbreak showed an identical banding pattern. We concluded that IS986 is an extremely suitable tool for the diagnosis and epidemiology of tuberculosis.

MeSH Terms
Animals Base Sequence DNA Probes DNA Transposable Elements Gene Rearrangement Guinea Pigs Humans Molecular Sequence Data Mycobacterium bovis/genetics Mycobacterium tuberculosis/genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Tuberculosis/diagnosis,epidemiology
Chemicals
DNA Probes DNA Transposable Elements
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hermans P W
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
van Soolingen D
Dale J W
Schuitema A R
McAdam R A
Catty D
van Embden J D
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.28.9.2051-2058.1990
Published
1990-09-00
Pages
2051-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC268102
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.28.9.2051-2058.1990 PMC Full Text View journal details
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