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PMID: 19796638 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Translation termination in pyrrolysine-utilizing archaea.

FEBS letters ·Vol. 583 ·No. 21 ·2009-11-03 ·Pages 3455-60

Alkalaeva E, Eliseev B, Ambrogelly A, Vlasov P, Kondrashov FA, Gundllapalli S, Frolova L, Söll D, Kisselev L

Abstract

Although some data link archaeal and eukaryotic translation, the overall mechanism of protein synthesis in archaea remains largely obscure. Both archaeal (aRF1) and eukaryotic (eRF1) single release factors recognize all three stop codons. The archaeal genus Methanosarcinaceae contains two aRF1 homologs, and also uses the UAG stop to encode the 22nd amino acid, pyrrolysine. Here we provide an analysis of the last stage of archaeal translation in pyrrolysine-utilizing species. We demonstrated that only one of two Methanosarcina barkeri aRF1 homologs possesses activity and recognizes all three stop codons. The second aRF1 homolog may have another unknown function. The mechanism of pyrrolysine incorporation in the Methanosarcinaceae is discussed.

MeSH Terms
Amino Acid Sequence Archaeal Proteins/chemistry,metabolism Codon, Terminator/genetics,metabolism Genome, Archaeal Humans Lysine/analogs & derivatives,metabolism Methanosarcinaceae/genetics,metabolism Molecular Sequence Data Peptide Termination Factors/chemistry,metabolism Phylogeny Protein Biosynthesis
Chemicals
Archaeal Proteins Codon, Terminator Peptide Termination Factors pyrrolysine Lysine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Alkalaeva Elena
Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences, Vavilov str. 32, Moscow 119991, Russia.
Eliseev Boris
Ambrogelly Alexandre
Vlasov Peter
Kondrashov Fyodor A
Gundllapalli Sharath
Frolova Lyudmila
Söll Dieter
Kisselev Lev
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Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
1873-3468
DOI
10.1016/j.febslet.2009.09.044
Published
2009-11-03
Epub
2009-00-29
Pages
3455-60
Language
English
Region
England
NLM ID
0155157
PMCID
PMC2857517
Subset
IM
Grants
NIGMS NIH HHS · R01 GM022854 · United States
NIGMS NIH HHS · R01 GM022854-33 · United States
External Links
PubMed source DOI: 10.1016/j.febslet.2009.09.044 PMC Full Text View journal details
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