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PMID: 1985210 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor Pu.1.

Journal of virology ·Vol. 65 ·No. 1 ·1991-01-00 ·Pages 464-7

Paul R, Schuetze S, Kozak SL, Kozak CA, Kabat D

Abstract

Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.1, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.

Related Genes
Sfpi-1 ets
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line DNA, Viral/genetics DNA-Binding Proteins/genetics Friend murine leukemia virus/genetics Genes, Viral Leukemia, Experimental/genetics,microbiology Mice Molecular Sequence Data Protein-Tyrosine Kinases/genetics Proviruses/genetics RNA, Messenger/genetics Restriction Mapping Retroviridae Proteins, Oncogenic/genetics Transcription Factors/genetics
Chemicals
DNA, Viral DNA-Binding Proteins RNA, Messenger Retroviridae Proteins, Oncogenic Transcription Factors oncogene proteins v-ets v-Spi-1 protein, Friend spleen focus-forming virus Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Paul R
Department of Biochemistry and Molecular Biology, School of Medicine, Oregon Health Sciences University, Portland 97201-3098.
Schuetze S
Kozak S L
Kozak C A
Kabat D
References (19)
19 references, click to expand
  1. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 PMID: 5277089
  2. Spi-1 oncogene activation in Rauscher and Friend murine virus-induced acute erythroleukemias.
    Leukemia. 1990 Jan;4(1):20-3 PMID: 2153262
  3. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  4. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  5. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
    Science. 1986 Oct 17;234(4774):364-8 PMID: 2876518
  6. Characterization and expression of the complementary DNA encoding rat histidine decarboxylase.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):733-7 PMID: 2300558
  7. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
    Cell. 1990 Apr 6;61(1):113-24 PMID: 2180582
  8. Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: regulatory role for the protooncogene ETS1.
    Proc Natl Acad Sci U S A. 1990 May;87(10):3723-7 PMID: 2187191
  9. The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.
    Oncogene. 1990 May;5(5):663-8 PMID: 1693183
  10. Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.
    Genes Dev. 1990 Apr;4(4):667-79 PMID: 2163347
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. Direct sequencing of denatured plasmid DNA.
    Methods Enzymol. 1987;152:556-62 PMID: 2443806
  13. Spi-1 is a putative oncogene in virally induced murine erythroleukaemias.
    Nature. 1988 Jan 21;331(6153):277-80 PMID: 2827041
  14. A tagged helper-free Friend virus causes clonal erythroblast immortality by specific proviral integration in the cellular genome.
    J Virol. 1988 Nov;62(11):4129-35 PMID: 2845127
  15. Mammalian ets-1 and ets-2 genes encode highly conserved proteins.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7862-6 PMID: 2847145
  16. Splenic accumulation of interleukin-3-dependent hematopoietic cells in Friend erythroleukemia.
    J Virol. 1989 Oct;63(10):4434-7 PMID: 2789294
  17. A common site for immortalizing proviral integrations in Friend erythroleukemia: molecular cloning and characterization.
    J Virol. 1989 Nov;63(11):4958-61 PMID: 2552176
  18. The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias.
    Oncogene. 1989 Dec;4(12):1449-56 PMID: 2594367
  19. The PU.1 transcription factor is the product of the putative oncogene Spi-1.
    Cell. 1990 Jun 29;61(7):1165-6 PMID: 2364426
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.1.464-467.1991
Published
1991-01-00
Pages
464-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240539
Subset
IM
Grants
NCI NIH HHS · CA25810 · United States
Databases
GENBANK
M38252, M63042, M63043, M63044, M63045, M63046, M63047, M63048, S50553, S50554
External Links
PubMed source DOI: 10.1128/JVI.65.1.464-467.1991 PMC Full Text View journal details
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