ANGULAR LIGHT-SCATTERING STUDIES ON ISOLATED MITOCHONDRIA.

The Journal of biophysical and biochemical cytology ·Vol. 10 ·No. 1 ·1961-05-01 ·Pages 15-21

Gotterer GS, Thompson TE, Lehninger AL

Abstract

Angular light-scattering studies have been carried out on suspensions of isolated rat liver mitochondria. The angular scatter pattern has a large forward component, typical of large particles. Changes in dissymmetry and in the intensity of light scattered at 90 degrees have been correlated with changes in optical density during the course of mitochondrial swelling and contraction. Such changes can be measured at mitochondrial concentrations much below those required for optical density measurements. Changes in mitochondrial geometry caused by factors "leaking" from mitochondria, not detectable by optical density measurements, have been demonstrated by measuring changes in dissymmetry. Angular light-scattering measurements therefore offer the advantages of increased sensitivity and of added indices of changes in mitochondrial conformation.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gotterer G S

The Department of Physiological Chemistry, The Johns Hopkins School of Medicine, Baltimore.

Thompson T E

Lehninger A L

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17 references, click to expand

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Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.10.1.15

Published

1961-05-01

Pages

15-21

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2225062

External Links

PubMed source DOI: 10.1083/jcb.10.1.15 PMC Full Text

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