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PMID: 19867297 Published · ppublish English Journal Article

Reactivation in vitro of immunocompetence in irradiated mouse spleen.

The Journal of experimental medicine ·Vol. 126 ·No. 2 ·1967-08-01 ·Pages 223-34

Globerson A, Auerbach R

Abstract

The restoration of immunological competence to irradiated adult mouse spleen was studied using an in vitro assay system. Competence of the spleen to evoke a graft versus host reaction in vitro was lost upon sublethal irradiation (550 R). Regeneration of immune competence occurred when such spleens were grown for 2-3 days in the presence of thymus tissue, but not when grown in the presence of a variety of other tissues. This thymic activity was demonstrated to occur across an intervening Millipore filter barrier. When lethal doses of irradiation were used, reactivation of immune competence did not occur unless both thymus and bone marrow were present. In this experimental situation the bone marrow appeared to supply the immunocompetent cells.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Globerson A
Department of Zoology, The University of Wisconsin, Madison, Wisconsin 53706.
Auerbach R
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.126.2.223
Published
1967-08-01
Pages
223-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138317
External Links
PubMed source DOI: 10.1084/jem.126.2.223 PMC Full Text View journal details
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