THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE.

The Journal of experimental medicine ·Vol. 73 ·No. 1 ·1941-01-01 ·Pages 109-23

Hobby GL, Dawson MH, Meyer K, Chaffee E

Abstract

A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65 degrees and 100 degrees C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration of the presence of hyaluronic acid in skin offers a plausible explanation for the mechanism of spreading on the basis of hyaluronidase activity. 2. Dissimilarities: (a) No parallelism was observed in the degree of activity of spreading factor and hyaluronidase in the same preparations. (b) All preparations which produced spreading did not contain hyaluronidase. (c) Antisera to hyaluronidase preparations specifically and completely inhibited the activity of the homologous enzyme but did not inhibit the spreading factor in the same preparations. The significance of the similarities and dissimilarities between the two reactions is discussed. It is concluded that while hyaluronidase may play a rôle in the spreading reaction the phenomenon is a complex one and cannot be explained on the basis of a simple chemical reaction.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hobby G L

Departments of Medicine and Ophthalmology, College of Physicians and Surgeons, Columbia University, the Edward Daniels Faulkner Arthritis Clinic, and the Institute of Ophthalmology, Presbyterian Hospital, New York.

Dawson M H

Meyer K

Chaffee E

References (9)

9 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.73.1.109

Published

1941-01-01

Pages

109-23

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2135114

External Links

PubMed source DOI: 10.1084/jem.73.1.109 PMC Full Text View journal details

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THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE.

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