The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

Nucleic acids research ·Vol. 4 ·No. 7 ·1977-07-00 ·Pages 2369-88

Van Kreijl CF, Bos JL

Abstract

The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethidium-induced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were use. The sequence consists of 66 consecutive AT base pairs flanked by two GC pairs and comprises nearly all of the mutant mitochondrial genome. The sequence, moreover, also represents the first part of wild-type mtDNA sequence so far.

MeSH Terms

Base Sequence DNA, Mitochondrial/genetics Ethidium/pharmacology Molecular Weight Mutation/drug effects Oligoribonucleotides/analysis Phosphoric Diester Hydrolases Ribonucleases Saccharomyces cerevisiae/drug effects,genetics Transcription, Genetic

Chemicals

DNA, Mitochondrial Oligoribonucleotides Ribonucleases Phosphoric Diester Hydrolases Ethidium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Van Kreijl C F

Bos J L

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.7.2369

Published

1977-07-00

Pages

2369-88

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342572

Subset

External Links

PubMed source DOI: 10.1093/nar/4.7.2369 PMC Full Text View journal details

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The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

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