Man-made antibodies.

Nature ·Vol. 349 ·No. 6307 ·1991-01-24 ·Pages 293-9

Winter G, Milstein C

Abstract

Monoclonal antibodies can now be genetically engineered and endowed with new properties. In the future, gene technology could enable antigen-binding fragments to be made by exploiting repertoires of variable domain genes derived from immunized animals and expressed in bacteria. How readily can this approach be extended to production of 'in vitro' repertoires of variable domain genes, and obviate the immunization of animals?

MeSH Terms

Animals Antibodies, Monoclonal/biosynthesis,chemistry,genetics,immunology Antibody Diversity Antigens/immunology Binding Sites, Antibody Gene Rearrangement Genes, Immunoglobulin Genetic Engineering Humans Hybridomas/immunology Immunization Immunoglobulin Variable Region/chemistry,immunology Macromolecular Substances Mice Molecular Structure Mutagenesis Protein Conformation Rats

Chemicals

Antibodies, Monoclonal Antigens Immunoglobulin Variable Region Macromolecular Substances

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Winter G

MRC Laboratory of Molecular Biology, Cambridge, UK.

Milstein C

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/349293a0

Published

1991-01-24

Pages

293-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/349293a0 View journal details

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