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PMID: 198799 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 9 ·1977-09-00 ·Pages 3715-9

Ross EM, Gilman AG

Abstract

Membranes of mouse L cells that contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] but lack beta-adrenergic receptors have been solubilized with Lubrol 12A9. Addition of such adenylate cyclase-containing extracts to beta-adrenergic receptor-replete membranes from adenylate cyclase-deficient S49 lymphoma cells results in the production of a catecholamine-sensitive adenylate cyclase system. The effects of beta-adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild-type S49 lymphoma cell. The uncoupled variant of the S49lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone-sensitive enzyme activity when added to adenylate cyclase-deficient membranes. Thus, the uncoupled and adenylate cyclase-deficient variants of the S49 cell are not complementary.

MeSH Terms
Adenylyl Cyclases/metabolism Binding, Competitive Catecholamines/pharmacology Cell Membrane/enzymology Fluorides/pharmacology Guanosine Triphosphate/pharmacology Guanylyl Imidodiphosphate/pharmacology Isoproterenol/pharmacology Kinetics L Cells/enzymology Mutation Receptors, Adrenergic/metabolism Receptors, Adrenergic, beta/metabolism
Chemicals
Catecholamines Receptors, Adrenergic Receptors, Adrenergic, beta Guanylyl Imidodiphosphate Guanosine Triphosphate Adenylyl Cyclases Isoproterenol Fluorides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ross E M
Gilman A G
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.9.3715
Published
1977-09-00
Pages
3715-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431700
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.9.3715 PMC Full Text View journal details
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