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PMID: 19881526 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Comparative genomic and phylogeographic analysis of Mycobacterium leprae.

Nature genetics ·Vol. 41 ·No. 12 ·2009-12-00 ·Pages 1282-9

Monot M, Honoré N, Garnier T, Zidane N, Sherafi D, Paniz-Mondolfi A, Matsuoka M, Taylor GM, Donoghue HD, Bouwman A, Mays S, Watson C, Lockwood D, Khamesipour A, Khamispour A, Dowlati Y, Jianping S, Rea TH, Vera-Cabrera L, Stefani MM, Banu S, Macdonald M, Sapkota BR, Spencer JS, Thomas J, Harshman K, Singh P, Busso P, Gattiker A, Rougemont J, Brennan PJ, Cole ST

Abstract

Reductive evolution and massive pseudogene formation have shaped the 3.31-Mb genome of Mycobacterium leprae, an unculturable obligate pathogen that causes leprosy in humans. The complete genome sequence of M. leprae strain Br4923 from Brazil was obtained by conventional methods (6x coverage), and Illumina resequencing technology was used to obtain the sequences of strains Thai53 (38x coverage) and NHDP63 (46x coverage) from Thailand and the United States, respectively. Whole-genome comparisons with the previously sequenced TN strain from India revealed that the four strains share 99.995% sequence identity and differ only in 215 polymorphic sites, mainly SNPs, and by 5 pseudogenes. Sixteen interrelated SNP subtypes were defined by genotyping both extant and extinct strains of M. leprae from around the world. The 16 SNP subtypes showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy.

MeSH Terms
Genes, Bacterial Genome, Bacterial Geography Humans Leprosy/genetics,microbiology Mycobacterium leprae/classification,genetics Phylogeny Polymorphism, Single Nucleotide Recombination, Genetic
Authors & Affiliations
32 authors, click to expand affiliations / ORCID
Monot Marc
Institut Pasteur, Paris, France.
Honoré Nadine
Garnier Thierry
Zidane Nora
Sherafi Diana
Paniz-Mondolfi Alberto
Matsuoka Masanori
Taylor G Michael
Donoghue Helen D
Bouwman Abi
Mays Simon
Watson Claire
Lockwood Diana
Khamesipour Ali
Khamispour Ali
Dowlati Yahya
Jianping Shen
Rea Thomas H
Vera-Cabrera Lucio
Stefani Mariane M
Banu Sayera
Macdonald Murdo
Sapkota Bishwa Raj
Spencer John S
Thomas Jérôme
Harshman Keith
Singh Pushpendra
Busso Philippe
Gattiker Alexandre
Rougemont Jacques
Brennan Patrick J
Cole Stewart T
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Article Info
Journal
Nature genetics
Abbr.
Nat Genet
ISSN
1546-1718
DOI
10.1038/ng.477
Published
2009-12-00
Epub
2009-00-01
Pages
1282-9
Language
English
Region
United States
NLM ID
9216904
Subset
IM
Grants
NIAID NIH HHS · N01-AI25469 · United States
NIAID NIH HHS · R01-AI47197-01A1 · United States
Corrections
ErratumIn
-
CommentIn
PMID: 19935762
External Links
PubMed source DOI: 10.1038/ng.477 View journal details
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