Abstract
The LKB1 tumor suppressor is a protein kinase that controls the activity of adenosine monophosphate-activated protein kinase (AMPK). LKB1 activity is regulated by the pseudokinase STRADalpha and the scaffolding protein MO25alpha through an unknown, phosphorylation-independent, mechanism. We describe the structure of the core heterotrimeric LKB1-STRADalpha-MO25alpha complex, revealing an unusual allosteric mechanism of LKB1 activation. STRADalpha adopts a closed conformation typical of active protein kinases and binds LKB1 as a pseudosubstrate. STRADalpha and MO25alpha promote the active conformation of LKB1, which is stabilized by MO25alpha interacting with the LKB1 activation loop. This previously undescribed mechanism of kinase activation may be relevant to understanding the evolution of other pseudokinases. The structure also reveals how mutations found in Peutz-Jeghers syndrome and in various sporadic cancers impair LKB1 function.
MeSH Terms
AMP-Activated Protein Kinase Kinases
AMP-Activated Protein Kinases/metabolism
Adaptor Proteins, Vesicular Transport/chemistry,metabolism
Allosteric Regulation
Amino Acid Sequence
Binding Sites
Calcium-Binding Proteins/chemistry,metabolism
Crystallography, X-Ray
Enzyme Activation
Humans
Models, Molecular
Molecular Sequence Data
Multiprotein Complexes/chemistry,metabolism
Mutant Proteins/chemistry,metabolism
Mutation
Phosphorylation
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
Protein Serine-Threonine Kinases/chemistry,metabolism
Protein Structure, Tertiary
Chemicals
Adaptor Proteins, Vesicular Transport
CAB39 protein, human
Calcium-Binding Proteins
Multiprotein Complexes
Mutant Proteins
STRADA protein, human
Protein Serine-Threonine Kinases
STK11 protein, human
AMP-Activated Protein Kinase Kinases
AMP-Activated Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zeqiraj Elton
Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland.
Filippi Beatrice Maria
Deak Maria
Alessi Dario R
van Aalten Daan M F
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