Optical interrogation of neural circuits in Caenorhabditis elegans.
The nematode Caenorhabditis elegans has a compact nervous system with only 302 neurons. Whereas most of the synaptic connections between these neurons have been identified by electron microscopy serial reconstructions, functional connections have been inferred between only a few neurons through combinations of electrophysiology, cell ablation, in vivo calcium imaging and genetic analysis. To map functional connections between neurons, we combined in vivo optical stimulation with simultaneous calcium imaging. We analyzed the connections from the ASH sensory neurons and RIM interneurons to the command interneurons AVA and AVD. Stimulation of ASH or RIM neurons using channelrhodopsin-2 (ChR2) resulted in activation of AVA neurons, evoking an avoidance behavior. Our results demonstrate that we can excite specific neurons expressing ChR2 while simultaneously monitoring G-CaMP fluorescence in several other neurons, making it possible to rapidly decipher functional connections in C. elegans neural circuits.
30 references, click to expand
-
Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution.PLoS One. 2007 Mar 21;2(3):e299 PMID: 17375185
-
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5 PMID: 14615590
-
EAT-4, a homolog of a mammalian sodium-dependent inorganic phosphate cotransporter, is necessary for glutamatergic neurotransmission in caenorhabditis elegans.J Neurosci. 1999 Jan 1;19(1):159-67 PMID: 9870947
-
Differential expression of glutamate receptor subunits in the nervous system of Caenorhabditis elegans and their regulation by the homeodomain protein UNC-42.J Neurosci. 2001 Mar 1;21(5):1510-22 PMID: 11222641
-
The neural circuit for touch sensitivity in Caenorhabditis elegans.J Neurosci. 1985 Apr;5(4):956-64 PMID: 3981252
-
A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein.Nat Biotechnol. 2001 Feb;19(2):137-41 PMID: 11175727
-
Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans.Neuron. 2000 Jun;26(3):583-94 PMID: 10896155
-
In vivo imaging of C. elegans ASH neurons: cellular response and adaptation to chemical repellents.EMBO J. 2005 Jan 12;24(1):63-72 PMID: 15577941
-
Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology.J Physiol. 2007 Jan 1;578(Pt 1):55-67 PMID: 17038427
-
Multimodal fast optical interrogation of neural circuitry.Nature. 2007 Apr 5;446(7136):633-9 PMID: 17410168
-
Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses.Curr Biol. 2005 Dec 20;15(24):2279-84 PMID: 16360690
-
G alphas-induced neurodegeneration in Caenorhabditis elegans.J Neurosci. 1998 Apr 15;18(8):2871-80 PMID: 9526004
-
Combinatorial expression of TRPV channel proteins defines their sensory functions and subcellular localization in C. elegans neurons.Neuron. 2002 Jul 18;35(2):307-18 PMID: 12160748
-
Optogenetic analysis of synaptic function.Nat Methods. 2008 Oct;5(10):895-902 PMID: 18794862
-
Low conductance gap junctions mediate specific electrical coupling in body-wall muscle cells of Caenorhabditis elegans.J Biol Chem. 2006 Mar 24;281(12):7881-9 PMID: 16434400
-
Light-sensitive neurons and channels mediate phototaxis in C. elegans.Nat Neurosci. 2008 Aug;11(8):916-22 PMID: 18604203
-
Laser killing of cells in Caenorhabditis elegans.Methods Cell Biol. 1995;48:225-50 PMID: 8531727
-
Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans.Nat Methods. 2007 Sep;4(9):727-31 PMID: 17704783
-
Active currents regulate sensitivity and dynamic range in C. elegans neurons.Neuron. 1998 Apr;20(4):763-72 PMID: 9581767
-
High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice.Proc Natl Acad Sci U S A. 2007 May 8;104(19):8143-8 PMID: 17483470
-
Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans.Cell. 1995 Oct 20;83(2):207-18 PMID: 7585938
-
The structure of the nervous system of the nematode Caenorhabditis elegans.Philos Trans R Soc Lond B Biol Sci. 1986 Nov 12;314(1165):1-340 PMID: 22462104
-
The genetics of Caenorhabditis elegans.Genetics. 1974 May;77(1):71-94 PMID: 4366476
-
A circuit for navigation in Caenorhabditis elegans.Proc Natl Acad Sci U S A. 2005 Mar 1;102(9):3184-91 PMID: 15689400
-
Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri.Nat Neurosci. 2008 Jun;11(6):631-3 PMID: 18432196
-
Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.Nature. 1997 Aug 28;388(6645):882-7 PMID: 9278050
-
A novel molecular solution for ultraviolet light detection in Caenorhabditis elegans.PLoS Biol. 2008 Aug 5;6(8):e198 PMID: 18687026
-
Neuronal substrates of complex behaviors in C. elegans.Annu Rev Neurosci. 2005;28:451-501 PMID: 16022603
-
Tyramine Functions independently of octopamine in the Caenorhabditis elegans nervous system.Neuron. 2005 Apr 21;46(2):247-60 PMID: 15848803
-
Glia are essential for sensory organ function in C. elegans.Science. 2008 Oct 31;322(5902):744-7 PMID: 18974354
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