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PMID: 1989881 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The MYC protein activates transcription of the alpha-prothymosin gene.

The EMBO journal ·Vol. 10 ·No. 1 ·1991-01-00 ·Pages 133-41

Eilers M, Schirm S, Bishop JM

Abstract

The proto-oncogene MYC encodes a nuclear protein whose biochemical and physiological functions remain uncertain. We used an estrogen-regulated version of the MYC protein to explore these functions. Activation of MYC in quiescent rat and mouse fibroblasts elicited re-entry into and progression through the cell cycle, bypassing early events that would follow stimulation of the cells with serum. Activation of MYC led to a rapid increase in transcription of the alpha-prothymosin gene, even in the absence of protein synthesis. We conclude that the product of MYC acts directly on transcription, in accord with inferences based on the structure of the MYC protein. The function of alpha-prothymosin is not known, but our results suggest that the protein may play a role in the proliferation of mammalian cells.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Cell Cycle Cell Line Cycloheximide/pharmacology DNA Replication Gene Expression Regulation/drug effects Gene Library Mice Molecular Sequence Data Protein Precursors/genetics Proto-Oncogene Proteins c-myc/metabolism Rats Thymidine/metabolism Thymosin/analogs & derivatives,genetics Transcription, Genetic
Chemicals
Actins Protein Precursors Proto-Oncogene Proteins c-myc prothymosin alpha Thymosin Cycloheximide Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eilers M
Department of Microbiology and Immunology, University of California-San Francisco 94143-0552.
Schirm S
Bishop J M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-01-00
Pages
133-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452621
Subset
IM
Databases
GENBANK
M55400, M55401, M55402, M65224, S60919, S60920, X55326, X59082, X59870, X59871
External Links
PubMed source PMC Full Text View journal details
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