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PMID: 19915075 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans.

Eukaryotic cell ·Vol. 9 ·No. 1 ·2010-01-00 ·Pages 224-6

Zhang C, Konopka JB

Abstract

Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.

MeSH Terms
Animals Candida albicans/cytology,metabolism Fluorescent Dyes/metabolism Fungal Proteins/genetics,metabolism Green Fluorescent Proteins/genetics,metabolism Humans Microscopy, Fluorescence/methods Recombinant Fusion Proteins/genetics,metabolism
Chemicals
Fluorescent Dyes Fungal Proteins Recombinant Fusion Proteins Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang Chengda
Graduate Program in Molecular and Cellular Biology, Stony Brook University, Stony Brook, New York 11794-5222, USA.
Konopka James B
References (9)
9 references, click to expand
  1. Septin function in yeast model systems and pathogenic fungi.
    Eukaryot Cell. 2005 Sep;4(9):1503-12 PMID: 16151244
  2. A guide to choosing fluorescent proteins.
    Nat Methods. 2005 Dec;2(12):905-9 PMID: 16299475
  3. Improved green fluorescent protein by molecular evolution using DNA shuffling.
    Nat Biotechnol. 1996 Mar;14(3):315-9 PMID: 9630892
  4. Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans.
    Microbiology (Reading). 1997 Feb;143 ( Pt 2):303-311 PMID: 9043107
  5. The green fluorescent protein.
    Annu Rev Biochem. 1998;67:509-44 PMID: 9759496
  6. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.
    J Bacteriol. 1999 Mar;181(6):1868-74 PMID: 10074081
  7. Expression of a chromosomally integrated, single-copy GFP gene in Candida albicans, and its use as a reporter of gene regulation.
    Mol Gen Genet. 1998 Feb;257(4):412-20 PMID: 9529522
  8. Cassettes for PCR-mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicans.
    Yeast. 2001 Jun 30;18(9):859-64 PMID: 11427968
  9. New pFA-cassettes for PCR-based gene manipulation in Candida albicans.
    J Basic Microbiol. 2006;46(5):416-29 PMID: 17009297
Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9786
DOI
10.1128/EC.00327-09
Published
2010-01-00
Epub
2009-00-13
Pages
224-6
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC2805285
Subset
IM
Grants
NIAID NIH HHS · R01 AI047837 · United States
NIAID NIH HHS · R01 AI47837 · United States
External Links
PubMed source DOI: 10.1128/EC.00327-09 PMC Full Text
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