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PMID: 19915088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The BTB/POZ domain of the Arabidopsis disease resistance protein NPR1 interacts with the repression domain of TGA2 to negate its function.

The Plant cell ·Vol. 21 ·No. 11 ·2009-11-00 ·Pages 3700-13

Boyle P, Le Su E, Rochon A, Shearer HL, Murmu J, Chu JY, Fobert PR, Després C

Abstract

TGA2 and NONEXPRESSER OF PR GENES1 (NPR1) are activators of systemic acquired resistance (SAR) and of the SAR marker gene pathogenesis-related-1 (PR-1) in Arabidopsis thaliana. TGA2 is a transcriptional repressor required for basal repression of PR-1, but during SAR, TGA2 recruits NPR1 as part of an enhanceosome. Transactivation by the enhanceosome requires the NPR1 BTB/POZ domain. However, the NPR1 BTB/POZ domain does not contain an autonomous transactivation domain; thus, its molecular role within the enhanceosome remains elusive. We now show by gel filtration analyses that TGA2 binds DNA as a dimer, tetramer, or oligomer. Using in vivo plant transcription assays, we localize the repression domain of TGA2 to the N terminus and demonstrate that this domain is responsible for modulating the DNA binding activity of the oligomer both in vitro and in vivo. We confirm that the NPR1 BTB/POZ domain interacts with and negates the molecular function of the TGA2 repression domain by excluding TGA2 oligomers from cognate DNA. These data distinguish the NPR1 BTB/POZ domain from other known BTB/POZ domains and establish its molecular role in the context of the Arabidopsis PR-1 gene enhanceosome.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Basic-Leucine Zipper Transcription Factors/chemistry,genetics,metabolism Chromatography, Gel DNA, Plant/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Enhancer Elements, Genetic/genetics Gene Expression Regulation, Plant/genetics Immunity, Innate/genetics Nuclear Proteins/chemistry,genetics,metabolism Organelles/genetics Protein Structure, Tertiary/genetics Repressor Proteins/genetics Transcriptional Activation/genetics
Chemicals
AHBP-1b protein, Arabidopsis Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors DNA, Plant DNA-Binding Proteins NPR1 protein, Arabidopsis Nuclear Proteins Repressor Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Boyle Patrick
Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.
Le Su Errol
Rochon Amanda
Shearer Heather L
Murmu Jhadeswar
Chu Jee Yan
Fobert Pierre R
Després Charles
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.109.069971
Published
2009-11-00
Epub
2009-00-13
Pages
3700-13
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2798319
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.109.069971 PMC Full Text View journal details
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