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PMID: 1993732 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus.

The Journal of cell biology ·Vol. 112 ·No. 4 ·1991-02-00 ·Pages 579-88

Donaldson JG, Lippincott-Schwartz J, Klausner RD

Abstract

The release of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A (BFA) action, preceding the movement of Golgi membrane into the ER. ATP depletion also causes the reversible redistribution of the 110-kD protein from Golgi membrane into the cytosol, although no Golgi disassembly occurs. To further define the effects of BFA on the association of the 110-kD protein with the Golgi apparatus we have used filter perforation techniques to produce semipermeable cells. All previously observed effects of BFA, including the rapid redistribution of the 110-kD protein and the movement of Golgi membrane into the ER, could be reproduced in the semipermeable cells. The role of guanine nucleotides in this process was investigated using the nonhydrolyzable analogue of GTP, GTP gamma S. Pretreatment of semipermeable cells with GTP gamma S prevented the BFA-induced redistribution of the 110-kD protein from the Golgi apparatus and movement of Golgi membrane into the ER. GTP gamma S could also abrogate the observed release of the 110-kD protein from Golgi membranes which occurred in response to ATP depletion. Additionally, when the 110-kD protein had first been dissociated from Golgi membranes by ATP depletion, GTP gamma S could restore Golgi membrane association of the 110-kD protein, but not if BFA was present. All of these effects observed with GTP gamma S in semipermeable cells could be reproduced in intact cells treated with AlF4-. These results suggest that guanine nucleotides regulate the dynamic association/dissociation of the 110-kD protein with the Golgi apparatus and that BFA perturbs this process by interfering with the association of the 110-kD protein with the Golgi apparatus.

MeSH Terms
Adenosine Triphosphate/physiology Aluminum/pharmacology Aluminum Compounds Animals Brefeldin A Cell Membrane Permeability Cells, Cultured Cyclopentanes/pharmacology Cytosol/metabolism Fluorides Fluorine/pharmacology Golgi Apparatus/drug effects,metabolism Guanine Nucleotides/physiology Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Membrane Proteins/metabolism Molecular Weight
Chemicals
Aluminum Compounds Cyclopentanes Guanine Nucleotides Membrane Proteins Brefeldin A tetrafluoroaluminate Fluorine Guanosine 5'-O-(3-Thiotriphosphate) Adenosine Triphosphate Aluminum Fluorides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Donaldson J G
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Lippincott-Schwartz J
Klausner R D
References (37)
37 references, click to expand
  1. Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):980-4 PMID: 6928695
  2. The cellular functions of small GTP-binding proteins.
    Science. 1990 Aug 10;249(4969):635-40 PMID: 2116664
  3. Assembly of tubulin with nucleotide analogs.
    J Biol Chem. 1981 Jan 10;256(1):106-11 PMID: 7451427
  4. Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4888-91 PMID: 6289322
  5. Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
    Cell. 1984 Dec;39(3 Pt 2):525-36 PMID: 6096009
  6. Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells.
    J Biol Chem. 1985 Jun 10;260(11):6654-62 PMID: 3922977
  7. Interorganelle transfer and glycosylation of yeast invertase in vitro.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2017-21 PMID: 2421286
  8. Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes.
    J Biol Chem. 1986 Aug 25;261(24):11398-403 PMID: 2426273
  9. A microtubule-binding protein associated with membranes of the Golgi apparatus.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2229-39 PMID: 3536963
  10. Reconstitution of transport of vesicular stomatitis virus G protein from the endoplasmic reticulum to the Golgi complex using a cell-free system.
    J Cell Biol. 1987 Mar;104(3):749-60 PMID: 3029144
  11. Protein localization and membrane traffic in yeast.
    Annu Rev Cell Biol. 1985;1:115-43 PMID: 3916315
  12. Brefeldin A arrests the intracellular transport of a precursor of complement C3 before its conversion site in rat hepatocytes.
    FEBS Lett. 1987 Apr 6;214(1):135-8 PMID: 3569512
  13. Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex.
    Cell. 1987 Aug 14;50(4):523-34 PMID: 3038335
  14. G proteins: transducers of receptor-generated signals.
    Annu Rev Biochem. 1987;56:615-49 PMID: 3113327
  15. Perforated MDCK cells support intracellular transport.
    EMBO J. 1987 Aug;6(8):2241-7 PMID: 3665874
  16. Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
    Cell. 1987 Dec 24;51(6):1053-62 PMID: 2826014
  17. Fluoride complexes of aluminium or beryllium act on G-proteins as reversibly bound analogues of the gamma phosphate of GTP.
    EMBO J. 1987 Oct;6(10):2907-13 PMID: 2826123
  18. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  19. Do GTPases direct membrane traffic in secretion?
    Cell. 1988 Jun 3;53(5):669-71 PMID: 2836065
  20. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast.
    Cell. 1988 Jun 3;53(5):753-68 PMID: 3131018
  21. ATP-dependent fusion of liposomes with the Golgi apparatus of perforated cells.
    Cell. 1988 Dec 2;55(5):797-805 PMID: 3191530
  22. Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum.
    J Biol Chem. 1988 Dec 5;263(34):18545-52 PMID: 3192548
  23. Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.
    Cell. 1989 Feb 10;56(3):357-68 PMID: 2536591
  24. Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
    Cell. 1989 Mar 10;56(5):801-13 PMID: 2647301
  25. Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.
    J Cell Biol. 1989 Apr;108(4):1245-56 PMID: 2538479
  26. Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum.
    J Cell Biol. 1989 Jul;109(1):61-72 PMID: 2745557
  27. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  28. Targeting and processing of glycophorins in murine erythroleukemia cells: use of brefeldin A as a perturbant of intracellular traffic.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6992-6 PMID: 2780556
  29. Biochemistry of interorganelle transport. A new frontier in enzymology emerges from versatile in vitro model systems.
    J Biol Chem. 1989 Oct 15;264(29):16965-8 PMID: 2507534
  30. A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2677-91 PMID: 2512296
  31. ADP-ribosylation factor is functionally and physically associated with the Golgi complex.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1238-42 PMID: 2105501
  32. Dynamic distribution of the Golgi marker thiamine pyrophosphatase is modulated by brefeldin A in rat hepatoma cells.
    Cell Struct Funct. 1989 Oct;14(5):605-16 PMID: 2559814
  33. Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway.
    Cell. 1990 Mar 9;60(5):821-36 PMID: 2178778
  34. Small GTP-binding protein associated with Golgi cisternae.
    Nature. 1990 Jun 7;345(6275):553-6 PMID: 2112230
  35. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2295-306 PMID: 2277061
  36. Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
    Cell. 1990 Jul 27;62(2):317-29 PMID: 2115402
  37. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
    Cell. 1980 Aug;21(1):205-15 PMID: 6996832
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.112.4.579
Published
1991-02-00
Pages
579-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288845
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.112.4.579 PMC Full Text View journal details
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