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PMID: 1996099 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.

Molecular and cellular biology ·Vol. 11 ·No. 3 ·1991-03-00 ·Pages 1372-81

Nuell MJ, Stewart DA, Walker L, Friedman V, Wood CM, Owens GA, Smith JR, Schneider EL, Dell' Orco R, Lumpkin CK

Abstract

Genes that act inside the cell to negatively regulate proliferation are of great interest because of their implications for such processes as development and cancer, but these genes have been difficult to clone. This report details the cloning and analysis of cDNA for prohibitin, a novel mammalian antiproliferative protein. Microinjection of synthetic prohibitin mRNA blocks entry into S phase in both normal fibroblasts and HeLa cells. Microinjection of an antisense oligonucleotide stimulates entry into S phase. By sequence comparison, the prohibitin gene appears to be the mammalian analog of Cc, a Drosophila gene that is vital for normal development.

Related Genes
Cd
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Cycle Cloning, Molecular DNA/biosynthesis,genetics Genes HeLa Cells Humans Liver/physiology Microinjections Molecular Sequence Data Prohibitins Protein Biosynthesis Proteins/genetics RNA, Messenger/genetics Rats Repressor Proteins Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Prohibitins Proteins RNA, Messenger Repressor Proteins DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Nuell M J
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, Maryland 21224.
Stewart D A
Walker L
Friedman V
Wood C M
Owens G A
Smith J R
Schneider E L
Dell' Orco R
Lumpkin C K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.3.1372-1381.1991
Published
1991-03-00
Pages
1372-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369408
Subset
IM
Grants
NIAAA NIH HHS · AA 07550-03 · United States
Databases
GENBANK
M35697, M35698, M35699, M35700, M61219, M73693, M73694, M73695, X52648, X52649
External Links
PubMed source DOI: 10.1128/mcb.11.3.1372-1381.1991 PMC Full Text View journal details
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