Home LiteratureArticle Details
PMID: 19962198 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Heat shock proteins on the human sperm surface.

Journal of reproductive immunology ·Vol. 84 ·No. 1 ·2010-01-00 ·Pages 32-40

Naaby-Hansen S, Herr JC

Abstract

The sperm plasma membrane is known to be critical to fertilization and to be highly regionalized into domains of head, mid- and principal pieces. However, the molecular composition of the sperm plasma membrane and its alterations during genital tract passage, capacitation and the acrosome reaction remains to be fully dissected. A two-dimensional gel-based proteomic study previously identified 98 human sperm proteins which were accessible for surface labelling with both biotin and radioiodine. In this report twelve dually labelled protein spots were excised from stained gels or PDVF membranes and analysed by mass spectrometry (MS) and Edman degradation. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP150), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP60), and several isoforms of the two testis-specific HSP70 chaperones HSPA2 and HSPA1L. An antiserum raised against the testis-specific HSPA2 chaperone reacted with three 65kDa HSPA2 isoforms and three high molecular weight surface proteins (78-79kDa, 84kDa and 90-93kDa). These proteins, together with seven 65kDa HSP70 forms, reacted with human anti-sperm IgG antibodies that blocked in vitro fertilization in humans. Three of these surface biotinylated human sperm antigens were immunoprecipitated with a rabbit antiserum raised against a linear peptide epitope in Chlamydia trachomatis HSP70. The results indicate diverse HSP chaperones are accessible for surface labelling on human sperm. Some of these share epitopes with C. trachomatis HSP70, suggesting an association between genital tract infection, immunity to HSP70 and reproductive failure.

MeSH Terms
Acrosome Reaction/immunology Animals Antigens/immunology Cell Membrane/metabolism Chlamydia Infections/immunology Endoplasmic Reticulum Chaperone BiP Epitopes/immunology Female Heat-Shock Proteins/chemistry,metabolism Humans Male Molecular Chaperones/chemistry,metabolism Rabbits Spermatozoa/chemistry,metabolism
Chemicals
Antigens Endoplasmic Reticulum Chaperone BiP Epitopes HSPA5 protein, human Heat-Shock Proteins Molecular Chaperones
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Naaby-Hansen Soren
Department of Clinical Immunology, Aalborg Sygehus, Aarhus University Hospital, Denmark. [email protected]
Herr John C
References (57)
57 references, click to expand
  1. Structural and functional diversities between members of the human HSPB, HSPH, HSPA, and DNAJ chaperone families.
    Biochemistry. 2008 Jul 8;47(27):7001-11 PMID: 18557634
  2. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  3. Hsp70 chaperones: cellular functions and molecular mechanism.
    Cell Mol Life Sci. 2005 Mar;62(6):670-84 PMID: 15770419
  4. Chlamydial serology in 1303 asymptomatic subfertile couples.
    Hum Reprod. 1997 Jul;12(7):1464-75 PMID: 9262279
  5. 150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells.
    Am J Physiol Cell Physiol. 2000 Jun;278(6):C1172-82 PMID: 10837345
  6. Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women.
    Biol Reprod. 1999 Jul;61(1):61-9 PMID: 10377032
  7. The ATPase domain of hsp70 possesses a unique binding specificity for 3'-sulfogalactolipids.
    J Biol Chem. 2001 Jan 5;276(1):449-56 PMID: 11024054
  8. Stress-induced phosphorylation of caveolin-1 and p38, and down-regulation of EGFr and ERK by the dietary lectin jacalin in two human carcinoma cell lines.
    Cell Stress Chaperones. 2006 Summer;11(2):135-47 PMID: 16817319
  9. Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition.
    J Cell Sci. 2004 Jul 15;117(Pt 16):3645-57 PMID: 15252132
  10. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5011-5 PMID: 8506346
  11. Hspa4l-deficient mice display increased incidence of male infertility and hydronephrosis development.
    Mol Cell Biol. 2006 Nov;26(21):8099-108 PMID: 16923965
  12. Relationship between an asymptomatic male genital tract exposure to Chlamydia trachomatis and an autoimmune response to spermatozoa.
    Hum Reprod. 1995 Nov;10(11):2952-5 PMID: 8747052
  13. Heat shock proteins form part of a danger signal cascade in response to lipopolysaccharide and GroEL.
    Clin Exp Immunol. 2006 Jul;145(1):183-9 PMID: 16792689
  14. Extracellular heat shock protein 70: a critical component for motoneuron survival.
    J Neurosci. 2005 Oct 19;25(42):9735-45 PMID: 16237177
  15. Analysis of chaperone proteins associated with human spermatozoa during capacitation.
    Mol Hum Reprod. 2007 Sep;13(9):605-13 PMID: 17595329
  16. Heat shock proteins in cancer: chaperones of tumorigenesis.
    Trends Biochem Sci. 2006 Mar;31(3):164-72 PMID: 16483782
  17. Immunolocalization of heat shock protein 70 (Hsp 70) in boar spermatozoa and its role during fertilization.
    Mol Reprod Dev. 2005 Dec;72(4):534-41 PMID: 16142794
  18. Immunoinfertility: a case study with implications for immunocontraception.
    Arch Androl. 1995 Jul-Aug;35(1):21-7 PMID: 8554427
  19. Previously undetected Chlamydia trachomatis infection, immunity to heat shock proteins and tubal occlusion in women undergoing in-vitro fertilization.
    Hum Reprod. 1999 Jan;14(1):60-4 PMID: 10374095
  20. Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein gene (HSPA2).
    Genomics. 1994 Sep 1;23(1):85-93 PMID: 7829106
  21. Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction.
    Biol Reprod. 2005 Feb;72(2):328-37 PMID: 15456702
  22. Expression of Hsp60 and Grp78 in the human endometrium and oviduct, and their effect on sperm functions.
    Hum Reprod. 2007 Oct;22(10):2606-14 PMID: 17670764
  23. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.
    Reprod Biol Endocrinol. 2003 Feb 14;1:27 PMID: 12646049
  24. Induction of heat shock proteins in B-cell exosomes.
    J Cell Sci. 2005 Aug 15;118(Pt 16):3631-8 PMID: 16046478
  25. The heat-shock proteins.
    Annu Rev Genet. 1988;22:631-77 PMID: 2853609
  26. An N-terminal truncated form of Orp150 is a cytoplasmic ligand for the anti-proliferative mushroom Agaricus bisporus lectin and is required for nuclear localization sequence-dependent nuclear protein import.
    J Biol Chem. 2002 Jul 5;277(27):24538-45 PMID: 11960996
  27. Male serum Chlamydia trachomatis IgA and IgG, but not heat shock protein 60 IgG, correlates with negatively affected semen characteristics and lower pregnancy rates in the infertile couple.
    Int J Androl. 2007 Apr;30(2):99-107 PMID: 17132153
  28. Demonstration of Chlamydia trachomatis IgG antibodies in the male partner of the infertile couple is correlated with a reduced likelihood of achieving pregnancy.
    Hum Reprod. 2004 May;19(5):1121-6 PMID: 15121732
  29. Molecular chaperones and protein quality control.
    Cell. 2006 May 5;125(3):443-51 PMID: 16678092
  30. The role of heat shock proteins in reproduction.
    Hum Reprod Update. 2000 Mar-Apr;6(2):149-59 PMID: 10782573
  31. Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function.
    J Biol Chem. 2003 Feb 28;278(9):7607-16 PMID: 12493773
  32. 150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death.
    J Biol Chem. 1999 Mar 5;274(10):6397-404 PMID: 10037731
  33. Genomic structure of the spermatid-specific hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man.
    J Biochem. 1998 Aug;124(2):347-53 PMID: 9685725
  34. "The stress of dying": the role of heat shock proteins in the regulation of apoptosis.
    J Cell Sci. 2004 Jun 1;117(Pt 13):2641-51 PMID: 15169835
  35. Outer dense fiber proteins are dominant postobstruction autoantigens in adult Lewis rats.
    Biol Reprod. 2001 May;64(5):1451-9 PMID: 11319151
  36. Role of heat shock protein HSP70-2 in spermatogenesis.
    Rev Reprod. 1999 Jan;4(1):23-30 PMID: 10051099
  37. Combined affinity labelling and mass spectrometry analysis of differential cell surface protein expression in normal and prostate cancer cells.
    Oncogene. 2005 Sep 1;24(38):5905-13 PMID: 16007208
  38. Large-scale and high-confidence proteomic analysis of human seminal plasma.
    Genome Biol. 2006;7(5):R40 PMID: 16709260
  39. Heat shock protein 70 (Hsp70): membrane location, export and immunological relevance.
    Methods. 2007 Nov;43(3):229-37 PMID: 17920520
  40. Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa.
    Biol Reprod. 1997 Mar;56(3):771-87 PMID: 9047024
  41. The effects of antibodies to heat shock protein 70 in fertilization and embryo development.
    Mol Hum Reprod. 2001 Sep;7(9):829-37 PMID: 11517289
  42. Extracellular heat shock proteins in cell signaling.
    FEBS Lett. 2007 Jul 31;581(19):3689-94 PMID: 17499247
  43. Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: role in gamete recognition and mycoplasma-related infertility.
    J Cell Physiol. 1995 Oct;165(1):7-17 PMID: 7559808
  44. Tyrosine phosphorylation of HSP-90 during mammalian sperm capacitation.
    Biol Reprod. 2003 Dec;69(6):1801-7 PMID: 12890735
  45. Expression of heat shock proteins by isolated mouse spermatogenic cells.
    Mol Cell Biol. 1988 Aug;8(8):3260-6 PMID: 3211143
  46. Characterization and cellular distribution of human spermatozoal heat shock proteins.
    Hum Reprod. 1992 May;7(5):637-45 PMID: 1639983
  47. Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.
    Mol Cell. 2002 Dec;10(6):1307-18 PMID: 12504007
  48. Sperm antibodies and human in vitro fertilization.
    Fertil Steril. 1988 Jun;49(6):1018-25 PMID: 3286287
  49. Putative creatine kinase M-isoform in human sperm is identifiedas the 70-kilodalton heat shock protein HspA2.
    Biol Reprod. 2000 Sep;63(3):925-32 PMID: 10952940
  50. Two-dimensional electrophoretic analysis of sperm antigens recognized by sperm immobilizing antibodies detected in infertile women.
    J Reprod Immunol. 2002 Jan;53(1-2):1-12 PMID: 11730899
  51. The 60 kDa heat shock protein in human semen: relationship with antibodies to spermatozoa and Chlamydia trachomatis.
    Hum Reprod. 1996 Dec;11(12):2600-3 PMID: 9021358
  52. Specific expression of heat shock protein HspA2 in human male germ cells.
    Mol Hum Reprod. 1999 Dec;5(12):1122-6 PMID: 10587366
  53. Humoral immune response to the chlamydial heat shock proteins hsp60 and hsp70 in Chlamydia-associated chronic salpingitis with tubal occlusion.
    Hum Reprod. 1996 Jun;11(6):1352-6 PMID: 8815069
  54. Characterization of spermatozoa surface antigens by antisperm antibodies and its influence on acrosomal exocytosis.
    Am J Reprod Immunol. 2003 Nov;50(5):411-9 PMID: 14750700
  55. Stress, heat shock proteins, and autoimmunity: how immune responses to heat shock proteins are to be used for the control of chronic inflammatory diseases.
    Ann N Y Acad Sci. 2007 Oct;1113:217-37 PMID: 17584980
  56. Post-obstruction rat sperm autoantigens identified by two-dimensional gel electrophoresis and western blotting.
    J Reprod Immunol. 1999 May;43(1):35-53 PMID: 10392780
  57. Immunolocalization of heat shock protein 70 in bovine spermatozoa.
    Andrologia. 2004 Oct;36(5):327-34 PMID: 15458553
Article Info
Journal
Journal of reproductive immunology
Abbr.
J Reprod Immunol
ISSN
1872-7603
DOI
10.1016/j.jri.2009.09.006
Email
[email protected]
Published
2010-01-00
Epub
2009-00-03
Pages
32-40
Language
English
Region
Ireland
NLM ID
8001906
PMCID
PMC2898571
Subset
IM
Grants
NCRR NIH HHS · P51 RR000166 · United States
NICHD NIH HHS · U54 HD029099-09 · United States
NICHD NIH HHS · U54 HD029099-070003 · United States
NICHD NIH HHS · U54 HD029099-08 · United States
NICHD NIH HHS · U54 HD029099-060003 · United States
NICHD NIH HHS · U54 HD29099 · United States
FIC NIH HHS · D43 TW000654-05 · United States
FIC NIH HHS · D43 TW000654 · United States
NICHD NIH HHS · U54 HD029099 · United States
NICHD NIH HHS · U54 HD029099-080003 · United States
NCRR NIH HHS · P51 RR000166-38S10110 · United States
NCRR NIH HHS · P51 RR000166-400110 · United States
External Links
PubMed source DOI: 10.1016/j.jri.2009.09.006 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2