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PMID: 1997207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein.

Cell ·Vol. 64 ·No. 4 ·1991-02-22 ·Pages 789-800

Cleves AE, McGee TP, Whitters EA, Champion KM, Aitken JR, Dowhan W, Goebl M, Bankaitis VA

Abstract

SEC14p is the yeast phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein, and it effects an essential stimulation of yeast Golgi secretory function. We now report that the SEC14p localizes to the yeast Golgi and that the SEC14p requirement can be specifically and efficiently bypassed by mutations in any one of at least six genes. One of these suppressor genes was the structural gene for yeast choline kinase (CKI), disruption of which rendered the cell independent of the normally essential SEC14p requirement. The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of the CDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated that SEC14p controls the phospholipid content of yeast Golgi membranes in vivo.

MeSH Terms
Carrier Proteins/genetics,metabolism Cloning, Molecular Cytidine Diphosphate Choline/metabolism Genes, Fungal Genes, Suppressor Genotype Golgi Apparatus/metabolism Membrane Proteins/genetics,metabolism Phosphatidylcholines/metabolism Phosphatidylinositols/metabolism Phospholipid Transfer Proteins Phospholipids/biosynthesis Saccharomyces cerevisiae/genetics,metabolism Suppression, Genetic
Chemicals
Carrier Proteins Membrane Proteins Phosphatidylcholines Phosphatidylinositols Phospholipid Transfer Proteins Phospholipids Cytidine Diphosphate Choline
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cleves A E
Department of Microbiology, University of Illinois, Urbana-Champaign 61801.
McGee T P
Whitters E A
Champion K M
Aitken J R
Dowhan W
Goebl M
Bankaitis V A
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(91)90508-v
Published
1991-02-22
Pages
789-800
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7802412
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044530 · United States
NIGMS NIH HHS · GM35143 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(91)90508-v PMC Full Text View journal details
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