Home LiteratureArticle Details
PMID: 1997430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Helicobacter felis gastritis in gnotobiotic rats: an animal model of Helicobacter pylori gastritis.

Infection and immunity ·Vol. 59 ·No. 3 ·1991-03-00 ·Pages 785-91

Fox JG, Lee A, Otto G, Taylor NS, Murphy JC

Abstract

The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had lymphocytes and eosinophils scattered in small foci throughout the subglandular region of the antrum. Small numbers of lymphocytes were present in the subglandular portion of the antral mucosa that focally extended through the lamina propria towards the luminal surface. Eight weeks postinoculation, the inflammation was confined to the antrum. It was characterized by increased numbers of lymphocytes and eosinophils in the subglandular areas, with focal aggregates of lymphocytes in the submucosa. Some lymphoid aggregates extended from the submucosa through the muscularis mucosa and lamina propria to the luminal surface. H. felis was demonstrated with the Warthin-Starry stain, bacterial culture, and urease assay, particularly in the antrum. H. felis also produced a significant immunoglobulin G antibody titer at 2, 4, and 8 weeks postinoculation as well as a transitory immunoglobulin M response at 2 to 4 weeks postinoculation. Contact control rats were not infected, inferring that fecal-oral spread of H. felis did not occur.

MeSH Terms
Animals Antibodies, Bacterial/immunology Colony Count, Microbial Disease Models, Animal Eosinophils Female Gastric Mucosa/immunology Gastritis/immunology,microbiology,pathology Germ-Free Life Gram-Negative Anaerobic Bacteria/immunology,pathogenicity Helicobacter Infections/immunology,microbiology,pathology Helicobacter pylori/immunology Immunoglobulin G/analysis Immunoglobulin M/analysis Lymphocytes Pyloric Antrum/immunology Rats
Chemicals
Antibodies, Bacterial Immunoglobulin G Immunoglobulin M
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fox J G
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
Lee A
Otto G
Taylor N S
Murphy J C
References (30)
30 references, click to expand
  1. Campylobacter-like organisms in chronic gastritis, peptic ulcer, and gastric carcinoma.
    Scand J Gastroenterol. 1987 Jun;22(5):553-8 PMID: 3629180
  2. Person-to-person transmission of Campylobacter pylori.
    Lancet. 1987 Sep 19;2(8560):680-1 PMID: 2887955
  3. Gastritis and Campylobacter pylori in healthy, asymptomatic volunteers.
    Arch Intern Med. 1988 May;148(5):1149-51 PMID: 3365082
  4. Naturally occurring chronic gastritis and C pylori infection in the rhesus monkey: a potential model for gastritis in man.
    Gut. 1988 Apr;29(4):465-72 PMID: 3371715
  5. Isolation of a spiral-shaped bacterium from the cat stomach.
    Infect Immun. 1988 Nov;56(11):2843-50 PMID: 3169989
  6. Prospective double-blind trial of duodenal ulcer relapse after eradication of Campylobacter pylori.
    Lancet. 1988 Dec 24-31;2(8626-8627):1437-42 PMID: 2904568
  7. Campylobacter pylori and peptic ulcer disease.
    Gastroenterology. 1989 Feb;96(2 Pt 2 Suppl):615-25 PMID: 2642447
  8. Campylobacter pylori-associated gastritis and immune response in a population at increased risk of gastric carcinoma.
    Am J Gastroenterol. 1989 Jul;84(7):775-81 PMID: 2741887
  9. Effect of a Campylobacter pylori protein on acid secretion by parietal cells.
    Lancet. 1989 Jul 22;2(8656):187-9 PMID: 2568521
  10. Seroepidemiology of Campylobacter pylori infection in various populations.
    J Clin Microbiol. 1989 Aug;27(8):1870-3 PMID: 2549098
  11. Gastric campylobacter-like organisms: their role in gastric disease of laboratory animals.
    Lab Anim Sci. 1989 Nov;39(6):543-53 PMID: 2687581
  12. Recurrence of duodenal ulcer and Campylobacter pylori infection after eradication.
    Med J Aust. 1989 Oct 16;151(8):431-5 PMID: 2687668
  13. Synergism between sodium chloride and sodium taurocholate and development of pepsinogen-altered pyloric glands: relevance to a medium-term bioassay system for gastric carcinogens and promoters in rats.
    Jpn J Cancer Res. 1989 Nov;80(11):1035-40 PMID: 2514164
  14. Cure of duodenal ulcer associated with eradication of Helicobacter pylori.
    Lancet. 1990 May 26;335(8700):1233-5 PMID: 1971318
  15. Helicobacter mustelae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans.
    Gastroenterology. 1990 Aug;99(2):352-61 PMID: 2365188
  16. Helicobacter pylori gastric infection in gnotobiotic beagle dogs.
    Infect Immun. 1990 Aug;58(8):2606-12 PMID: 2370111
  17. A small animal model of human Helicobacter pylori active chronic gastritis.
    Gastroenterology. 1990 Nov;99(5):1315-23 PMID: 2210240
  18. Helicobacter pylori and gastric carcinoma. Serum antibody prevalence in populations with contrasting cancer risks.
    Cancer. 1990 Dec 15;66(12):2569-74 PMID: 2249197
  19. Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria.
    Int J Syst Bacteriol. 1991 Jan;41(1):31-8 PMID: 1704791
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Ultrastructure of a spiraled microorganism in the gastric mucosa of dogs.
    Am J Vet Res. 1970 Aug;31(8):1453-62 PMID: 5449901
  22. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.
    Lancet. 1984 Jun 16;1(8390):1311-5 PMID: 6145023
  23. Effects of sodium chloride, saccharin, phenobarbital and aspirin on gastric carcinogenesis in rats after initiation with N-methyl-N'-nitro-N-nitrosoguanidine.
    Gan. 1984 Jun;75(6):494-501 PMID: 6468837
  24. Flow cytometric analysis of the effect of sodium chloride on gastric cancer risk in the rat.
    Cancer Res. 1985 Nov;45(11 Pt 2):5608-16 PMID: 4053034
  25. Campylobacter-like organisms isolated from gastric mucosa of ferrets.
    Am J Vet Res. 1986 Feb;47(2):236-9 PMID: 3954197
  26. Campylobacter-like organisms in association with symptomatic gastritis in children.
    J Pediatr. 1986 Jul;109(1):80-3 PMID: 3723244
  27. Ultrastructure of the gastric mucosa harboring Campylobacter-like organisms.
    Am J Clin Pathol. 1986 Nov;86(5):575-82 PMID: 2430450
  28. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH.
    Am J Gastroenterol. 1987 Mar;82(3):192-9 PMID: 3826027
  29. Campylobacter pyloridis gastritis I: Detection of urease as a marker of bacterial colonization and gastritis.
    Am J Gastroenterol. 1987 Apr;82(4):292-6 PMID: 2436470
  30. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet.
    Infect Immun. 1987 Nov;55(11):2789-96 PMID: 3666963
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.3.785-791.1991
Published
1991-03-00
Pages
785-91
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258328
Subset
IM
Grants
NCI NIH HHS · P01-CA-26731 · United States
PHS HHS · R01-A125631 · United States
NCRR NIH HHS · RR01046-14 · United States
External Links
PubMed source DOI: 10.1128/iai.59.3.785-791.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2