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PMID: 1999457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a novel nuclear domain.

The Journal of cell biology ·Vol. 112 ·No. 5 ·1991-03-00 ·Pages 785-95

Ascoli CA, Maul GG

Abstract

For most known nuclear domains (ND), specific functions have been identified. In this report we used murine mAbs and human autoantibodies to investigate precisely circumscribed structures 0.2-0.3 micron in diameter which appear as "nuclear dots" distributed throughout the nucleoplasm. Nuclear dots are metabolically stable and resistant to nuclease digestion and salt extraction. The localization of nuclear dots is separate from kinetochores, centromeres, sites of mRNA processing and tRNA synthesis, nuclear bodies, and chromosomes. The nuclear dots, therefore, represent a novel ND. Nuclear dots break down as cells enter metaphase and reassemble at telophase. In interphase cells, nuclear dots are frequently "paired," and some are visible as "doublets" when stained with one particular antiserum. The number of dot doublets increased when quiescent cells were stimulated with serum although the total number of dots did not change substantially. One of the antigens was identified as a protein with a molecular mass of approximately 55 kD showing three charge isomers in the pI range of 7.4 to 7.7. Autoantibodies affinity purified from this nuclear dot protein (NDP-55) show nuclear dots exclusively. Nuclear dot-negative rat liver parenchymal cells became positive after chemical hepatectomy, suggesting involvement of the NDP-55 in the proliferative state of cells.

MeSH Terms
Amanitins/pharmacology Animals Autoantibodies Cell Cycle Cell Line Cell Nucleus/chemistry,ultrastructure Cycloheximide/pharmacology Fluorescent Antibody Technique Humans Immunoblotting Isoelectric Focusing Liver/chemistry,ultrastructure Liver Regeneration Nuclear Proteins/analysis,immunology Puromycin/pharmacology Rats Rats, Inbred Strains Tumor Cells, Cultured
Chemicals
Amanitins Autoantibodies Nuclear Proteins Puromycin Cycloheximide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ascoli C A
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Maul G G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.112.5.785
Published
1991-03-00
Pages
785-95
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288866
Subset
IM
Grants
NIAMS NIH HHS · AR-38907 · United States
NCI NIH HHS · CA-10815 · United States
External Links
PubMed source DOI: 10.1083/jcb.112.5.785 PMC Full Text View journal details
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