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PMID: 19998274 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells.

Hepatology (Baltimore, Md.) ·Vol. 51 ·No. 1 ·2010-01-00 ·Pages 297-305

Si-Tayeb K, Noto FK, Nagaoka M, Li J, Battle MA, Duris C, North PE, Dalton S, Duncan SA

Abstract

There exists a worldwide shortage of donor livers available for orthotropic liver transplantation and hepatocyte transplantation therapies. In addition to their therapeutic potential, primary human hepatocytes facilitate the study of molecular and genetic aspects of human hepatic disease and development and provide a platform for drug toxicity screens and identification of novel pharmaceuticals with potential to treat a wide array of metabolic diseases. The demand for human hepatocytes, therefore, heavily outweighs their availability. As an alternative to using donor livers as a source of primary hepatocytes, we explored the possibility of generating patient-specific human hepatocytes from induced pluripotent stem (iPS) cells. We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe a procedure that facilitates the efficient generation of highly differentiated human hepatocyte-like cells from iPS cells that display key liver functions and can integrate into the hepatic parenchyma in vivo.

MeSH Terms
Animals Cell Differentiation/physiology Hepatocytes/transplantation Humans Induced Pluripotent Stem Cells/physiology Mice
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Si-Tayeb Karim
Department of Cell Biology, Division of Pediatric Pathology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Noto Fallon K
Nagaoka Masato
Li Jixuan
Battle Michele A
Duris Christine
North Paula E
Dalton Stephen
Duncan Stephen A
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Article Info
Journal
Hepatology (Baltimore, Md.)
Abbr.
Hepatology
ISSN
1527-3350
DOI
10.1002/hep.23354
Published
2010-01-00
Pages
297-305
Language
English
Region
United States
NLM ID
8302946
PMCID
PMC2946078
Subset
IM
Grants
NIDDK NIH HHS · R01 DK055743 · United States
NHLBI NIH HHS · P01 HL089471-01A16834 · United States
NIDDK NIH HHS · R01 DK055743-10 · United States
NIGMS NIH HHS · P01 GM085354 · United States
NIDDK NIH HHS · RC1 DK087377 · United States
NHLBI NIH HHS · P01 HL089471-01A16838 · United States
NIDDK NIH HHS · RC1 DK087377-01 · United States
NHLBI NIH HHS · P01 HL089471 · United States
Corrections
ErratumIn
-
CommentIn
PMID: 19957368
CommentIn
PMID: 20034034
External Links
PubMed source DOI: 10.1002/hep.23354 PMC Full Text View journal details
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