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PMID: 2000372 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Role of integration host factor in the regulation of the glnHp2 promoter of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 5 ·1991-03-01 ·Pages 1631-5

Claverie-Martin F, Magasanik B

Abstract

The glnHPQ operon of Escherichia coli encodes components of the high-affinity glutamine transport system. One of the two promoters of this operon, glnHp2, is responsible for expression of the operon under nitrogen-limiting conditions. The general nitrogen regulatory protein (NRI) binds to two overlapping sites centered at -109 and -122 from the transcription start site and, when phosphorylated, activates transcription of glnHp2 by catalyzing isomerization of the closed sigma 54-RNA polymerase promoter complex to an open complex. The DNA-bending protein integration host factor (IHF) binds to a site immediately upstream of glnHp2 and enhances the activation of open complex formation by NRI phosphate. The NRI-binding sites can be moved several hundred base pairs further upstream without altering the ability of NRI phosphate to activate open complex formation. We propose that the IHF-induced bend can facilitate or obstruct the interaction between NRI phosphate and the closed complex depending on the relative positions of NRI phosphate and sigma 54-RNA polymerase on the DNA.

Related Genes
glnHp2
MeSH Terms
Bacterial Proteins Base Sequence DNA Nucleotidyltransferases/metabolism DNA-Binding Proteins/genetics DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Glutamine/metabolism Integrases Molecular Sequence Data Oligonucleotide Probes PII Nitrogen Regulatory Proteins Plasmids Promoter Regions, Genetic Protein Binding Restriction Mapping Sigma Factor/genetics Trans-Activators Transcription Factors/genetics Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Oligonucleotide Probes PII Nitrogen Regulatory Proteins Sigma Factor Trans-Activators Transcription Factors glnG protein, E coli Glutamine DNA Nucleotidyltransferases Integrases DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Claverie-Martin F
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Magasanik B
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.5.1631
Published
1991-03-01
Pages
1631-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51078
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.5.1631 PMC Full Text View journal details
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