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PMID: 2000384 Published · ppublish English Journal Article

Heme inhibits human immunodeficiency virus 1 replication in cell cultures and enhances the antiviral effect of zidovudine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 5 ·1991-03-01 ·Pages 1756-9

Levere RD, Gong YF, Kappas A, Bucher DJ, Wormser GP, Abraham NG

Abstract

The effects of heme alone and heme administered together with 3'-azido-3'-deoxythymidine (AZT) on human immunodeficiency virus replication in human peripheral blood lymphocytes and in the H9 cell line were studied. Heme enhanced the antiviral action of AZT against both drug-resistant and drug-sensitive viral strains; the heme effect was more pronounced against the latter. Moreover, heme alone displayed a significant ability to inhibit viral replication in concentrations markedly smaller than those required to inhibit the reverse transcriptase of Rauscher murine leukemia virus. The results of this study extend the range of pharmacological actions that metalloporphyrins exert in biological systems and suggest that further study of the interactions of the natural compound heme and human immunodeficiency virus chemotherapeutic agents such as AZT may be useful.

MeSH Terms
Antiviral Agents Cell Line Cells, Cultured Dose-Response Relationship, Drug Drug Resistance, Microbial Drug Synergism HIV-1/drug effects,isolation & purification,physiology Heme/pharmacology Humans Kinetics Lymphocytes/microbiology Virus Replication/drug effects Zidovudine/pharmacology
Chemicals
Antiviral Agents Heme Zidovudine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Levere R D
Laboratory of Metabolism and Pharmacology, Rockefeller University Hospital, New York, NY 10021.
Gong Y F
Kappas A
Bucher D J
Wormser G P
Abraham N G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.5.1756
Published
1991-03-01
Pages
1756-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51103
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.5.1756 PMC Full Text View journal details
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