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PMID: 20008169 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Physiological versatility of the extremely thermoacidophilic archaeon Metallosphaera sedula supported by transcriptomic analysis of heterotrophic, autotrophic, and mixotrophic growth.

Applied and environmental microbiology ·Vol. 76 ·No. 3 ·2010-02-00 ·Pages 931-5

Auernik KS, Kelly RM

Abstract

Comparative transcriptomic analysis of autotrophic, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula (70 degrees C, pH 2.0) revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-hydroxybutyrate pathway and implicated a membrane-bound hydrogenase (Msed_0944-Msed_0946) for growth on H(2). Routes for generation of ATP and reducing equivalents were also identified.

MeSH Terms
Aerobiosis/genetics Archaeal Proteins/genetics,metabolism Autotrophic Processes Carbon Dioxide/metabolism Carbon Isotopes Gene Expression Profiling Gene Expression Regulation, Archaeal Heterotrophic Processes Hot Temperature Hydroxybutyrates/metabolism Oligonucleotide Array Sequence Analysis Substrate Specificity/genetics Sulfolobaceae/genetics,growth & development,metabolism Temperature
Chemicals
Archaeal Proteins Carbon Isotopes Hydroxybutyrates Carbon Dioxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Auernik Kathryne S
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Kelly Robert M
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24 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.01336-09
Published
2010-02-00
Epub
2009-00-11
Pages
931-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2813022
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.01336-09 PMC Full Text View journal details
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