Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

Journal of microbiological methods ·第 80 卷 ·第 2 期 ·2010-03-30

Lord Jeffrey C, Hartzer Kris, Toutges Michelle, Oppert Brenda

摘要

To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.

文献信息

期刊

Journal of microbiological methods

期刊简称

J Microbiol Methods

ISSN

0167-7012

DOI

10.1016/j.mimet.2009.12.007

发表日期

2010-03-30

收录日期

2010-02-01

更新日期

2010-02-01

语言

英语

国家/地区

Netherlands

NLM ID

8306883

外部链接

PubMed 原文 DOI: 10.1016/j.mimet.2009.12.007 查看期刊详情

分析服务

Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

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