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PMID: 20037591 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals.

Nature methods ·Vol. 7 ·No. 2 ·2010-02-00 ·Pages 126-9

Clark JJ, Sandberg SG, Wanat MJ, Gan JO, Horne EA, Hart AS, Akers CA, Parker JG, Willuhn I, Martinez V, Evans SB, Stella N, Phillips PE

Abstract

Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.

MeSH Terms
Animals Behavior, Animal/physiology Biosensing Techniques/instrumentation Dopamine/analysis Electrochemistry/instrumentation Electrodes, Implanted Equipment Design Equipment Failure Analysis Mesencephalon/physiology Mice Microelectrodes Miniaturization Monitoring, Ambulatory/instrumentation Neurotransmitter Agents/analysis Rats Reproducibility of Results Sensitivity and Specificity
Chemicals
Neurotransmitter Agents Dopamine
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Clark Jeremy J
Department of Psychiatry and Behavioral Sciences and Department of Pharmacology, University of Washington, Seattle, Washington, USA.
Sandberg Stefan G
Wanat Matthew J
Gan Jerylin O
Horne Eric A
Hart Andrew S
Akers Christina A
Parker Jones G
Willuhn Ingo
Martinez Vicente
Evans Scott B
Stella Nephi
Phillips Paul E M
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.1412
Published
2010-02-00
Epub
2009-00-27
Pages
126-9
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2849934
Subset
IM
Grants
NIAAA NIH HHS · T32 AA007455-25 · United States
NIDA NIH HHS · R21 DA024140 · United States
NIAAA NIH HHS · T32 AA007455 · United States
NIDA NIH HHS · R01 DA014486 · United States
NIAAA NIH HHS · T32-AA007455 · United States
NIDA NIH HHS · R21 DA021793 · United States
NIDA NIH HHS · T32 DA007278-05 · United States
NIMH NIH HHS · R01-MH079292 · United States
NIDA NIH HHS · R01 DA014486-01 · United States
NIDA NIH HHS · T32-DA007278 · United States
NIMH NIH HHS · R01 MH079292-01A1 · United States
NIDA NIH HHS · F32-DA024540 · United States
NIDA NIH HHS · R01-DA014486 · United States
NIDA NIH HHS · R21 DA024140-01 · United States
NIDA NIH HHS · T32 DA007278 · United States
NIDA NIH HHS · F32 DA024540 · United States
NIDA NIH HHS · R21 DA021793-01A1 · United States
NIMH NIH HHS · R01 MH079292 · United States
NIGMS NIH HHS · T32 GM007270 · United States
NIGMS NIH HHS · T32 GM007270-24 · United States
NIDA NIH HHS · F32 DA024540-01 · United States
NIGMS NIH HHS · T32-GM007270 · United States
NIDA NIH HHS · R21-DA024140 · United States
Corrections
CommentIn
PMID: 20111035
External Links
PubMed source DOI: 10.1038/nmeth.1412 PMC Full Text View journal details
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