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PMID: 2004832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heat shock response of spirochetes.

Infection and immunity ·Vol. 59 ·No. 4 ·1991-04-00 ·Pages 1572-5

Stamm LV, Gherardini FC, Parrish EA, Moomaw CR

Abstract

We examined the heat shock response of the pathogenic spirochetes Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans and certain saprophytic spirochetes. Cellular proteins synthesized after shifts to higher temperatures were [35S]methionine labeled and analyzed by gel electrophoresis and fluorography. Only T. pallidum failed to exhibit an obvious heat shock response. GroEL and DnaK homologs were identified in the various species, although these proteins were not thermoinducible in T. pallidum or Treponema denticola. DNA hybridization studies indicate that spirochetal groEL and dnaK genes are highly conserved.

MeSH Terms

Borrelia burgdorferi Group/genetics,metabolism Heat-Shock Proteins/biosynthesis,genetics Leptospira interrogans/genetics,metabolism Treponema pallidum/genetics,metabolism

Chemicals

Heat-Shock Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Stamm L V

Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400.

Gherardini F C

Parrish E A

Moomaw C R

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.59.4.1572-1575.1991

Published

1991-04-00

Pages

1572-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC257880

Subset

Grants

NIAID NIH HHS · AI15036 · United States

NIAID NIH HHS · AI24976 · United States

External Links

PubMed source DOI: 10.1128/iai.59.4.1572-1575.1991 PMC Full Text View journal details

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