Home LiteratureArticle Details
PMID: 2005910 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a telomere-binding protein from Physarum polycephalum.

Molecular and cellular biology ·Vol. 11 ·No. 4 ·1991-04-00 ·Pages 2282-90

Coren JS, Epstein EM, Vogt VM

Abstract

We have partially purified a nuclear protein (PPT) from Physarum polycephalum that binds to the extrachromosomal ribosomal DNA telomeres of this acellular slime mold. Binding is specific for the (T2AG3)n telomere repeats, as evidenced by nitrocellulose filter binding assays, by gel mobility shift assays with both DNA fragments and double-stranded oligonucleotides, and by DNase I footprinting. PPT is remarkably heat stable, showing undiminished binding activity after incubation at 90 degrees C. It sediments at 1.2S, corresponding to a molecular weight of about 10,000 (for a globular protein), and its binding activity is undiminished by incubation with RNase, suggesting that it is not a ribonucleoprotein. We hypothesize that PPT plays a structural role in telomeres, perhaps preventing nucleolytic degradation or promoting telomere extension by a telomere-specific terminal transferase.

MeSH Terms
Base Sequence Chromosomes, Fungal/metabolism DNA-Binding Proteins/chemistry,isolation & purification,metabolism Deoxyribonuclease I/metabolism Electrophoresis, Polyacrylamide Gel Fungal Proteins/chemistry,isolation & purification,metabolism Molecular Sequence Data Molecular Weight Physarum/metabolism Repetitive Sequences, Nucleic Acid
Chemicals
DNA-Binding Proteins Fungal Proteins Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Coren J S
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
Epstein E M
Vogt V M
References (55)
55 references, click to expand
  1. Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs.
    Cell. 1987 Dec 24;51(6):899-908 PMID: 3690664
  2. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity.
    Cell. 1987 Dec 24;51(6):887-98 PMID: 3319189
  3. Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis.
    Nature. 1988 Jul 28;334(6180):364-6 PMID: 3393228
  4. Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules.
    Nucleic Acids Res. 1988 Jul 25;16(14B):6953-72 PMID: 3136437
  5. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6 PMID: 3413114
  6. The binding in vitro of the intermediate filament protein vimentin to synthetic oligonucleotides containing telomere sequences.
    J Biol Chem. 1988 Dec 15;263(35):18744-9 PMID: 3264281
  7. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis.
    Nature. 1989 Jan 26;337(6205):331-7 PMID: 2463488
  8. Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.
    Mol Cell Biol. 1988 Dec;8(12):5086-99 PMID: 3072472
  9. Lac repressor-operator interaction. I. Equilibrium studies.
    J Mol Biol. 1970 Feb 28;48(1):67-83 PMID: 4915295
  10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  11. Structure of ribosomal DNA in Physarum polycephalum.
    J Mol Biol. 1976 Sep 25;106(3):567-87 PMID: 988186
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. Non-nucleosomal packaging of a tandemly repeated DNA sequence at termini of extrachromosomal DNA coding for rRNA in Tetrahymena.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2263-7 PMID: 6941283
  14. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3015-9 PMID: 6265931
  15. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  16. Protein tightly bound near the termini of the Physarum extrachromosomal rDNA palindrome.
    J Cell Biol. 1981 Oct;91(1):309-14 PMID: 7298725
  17. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  18. Structure of the central spacer region of extrachromosomal ribosomal DNA in Physarum polycephalum.
    J Mol Biol. 1982 Aug 15;159(3):359-81 PMID: 6300409
  19. Identification of a telomeric DNA sequence in Trypanosoma brucei.
    Cell. 1984 Feb;36(2):447-57 PMID: 6319025
  20. Elaboration of telomeres in yeast: recognition and modification of termini from Oxytricha macronuclear DNA.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1475-9 PMID: 6324194
  21. DNA sequences of telomeres maintained in yeast.
    Nature. 1984 Jul 12-18;310(5973):154-7 PMID: 6330571
  22. The molecular structure of centromeres and telomeres.
    Annu Rev Biochem. 1984;53:163-94 PMID: 6383193
  23. CDC17: an essential gene that prevents telomere elongation in yeast.
    Cell. 1985 Aug;42(1):249-57 PMID: 3893744
  24. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  25. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
    Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
  26. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.
    Cell. 1985 Dec;43(2 Pt 1):405-13 PMID: 3907856
  27. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  28. Nucleotide sequence of the central non-transcribed spacer region of Physarum polycephalum rDNA.
    Gene. 1985;39(2-3):203-11 PMID: 3005112
  29. Properties of the telomeric DNA-binding protein from Oxytricha nova.
    Biochemistry. 1989 Jan 24;28(2):769-74 PMID: 2713343
  30. A mutant with a defect in telomere elongation leads to senescence in yeast.
    Cell. 1989 May 19;57(4):633-43 PMID: 2655926
  31. Oxytricha telomeric nucleoprotein complexes reconstituted with synthetic DNA.
    Nucleic Acids Res. 1989 Jun 12;17(11):4235-53 PMID: 2500641
  32. Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers.
    Mol Cell Biol. 1989 Jun;9(6):2761-4 PMID: 2474761
  33. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats.
    Cell. 1989 Nov 3;59(3):521-9 PMID: 2805070
  34. Assembly and self-association of oxytricha telomeric nucleoprotein complexes.
    Cell. 1989 Nov 17;59(4):719-28 PMID: 2582492
  35. Monovalent cation-induced structure of telomeric DNA: the G-quartet model.
    Cell. 1989 Dec 1;59(5):871-80 PMID: 2590943
  36. Functional evidence for an RNA template in telomerase.
    Science. 1990 Feb 2;247(4942):546-52 PMID: 1689074
  37. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs.
    Nature. 1990 Mar 8;344(6262):126-32 PMID: 1689810
  38. A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1.
    Curr Genet. 1989 Oct;16(4):225-39 PMID: 2697465
  39. A sodium-potassium switch in the formation of four-stranded G4-DNA.
    Nature. 1990 Mar 29;344(6265):410-4 PMID: 2320109
  40. Telomere-telomere recombination provides an express pathway for telomere acquisition.
    Nature. 1990 May 31;345(6274):456-8 PMID: 2111466
  41. Characterization of telomere DNA from Neurospora crassa.
    Gene. 1990 Apr 16;88(2):159-65 PMID: 1971801
  42. Telomere structure in Euplotes crassus: characterization of DNA-protein interactions and isolation of a telomere-binding protein.
    Mol Cell Biol. 1990 Jul;10(7):3421-31 PMID: 2355912
  43. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability.
    Cell. 1990 Nov 16;63(4):739-50 PMID: 2225074
  44. Involvement of the silencer and UAS binding protein RAP1 in regulation of telomere length.
    Science. 1990 Oct 26;250(4980):549-53 PMID: 2237406
  45. A yeast protein that binds to vertebrate telomeres and conserved yeast telomeric junctions.
    Genes Dev. 1991 Jan;5(1):49-59 PMID: 1989906
  46. ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.
    J Bacteriol. 1963 Aug;86:324-31 PMID: 14058960
  47. Structure and function of telomeres.
    Annu Rev Genet. 1989;23:579-604 PMID: 2694944
  48. Identification of yeast mutants with altered telomere structure.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1398-402 PMID: 3513174
  49. Identification of a telomere-binding activity from yeast.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3713-7 PMID: 3520552
  50. Telomere proteins: specific recognition and protection of the natural termini of Oxytricha macronuclear DNA.
    Cell. 1986 Oct 24;47(2):195-205 PMID: 3094961
  51. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  52. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements.
    Cell. 1987 Dec 4;51(5):721-32 PMID: 3315231
  53. The cohering telomeres of Oxytricha.
    Nucleic Acids Res. 1987 Nov 11;15(21):8877-98 PMID: 3120149
  54. Identification of the telomeric sequence of the acellular slime molds Didymium iridis and Physarum polycephalum.
    Nucleic Acids Res. 1987 Nov 25;15(22):9143-52 PMID: 3684591
  55. Telomeric DNA-protein interactions of Oxytricha macronuclear DNA.
    Genes Dev. 1987 Oct;1(8):783-93 PMID: 3123321
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.4.2282-2290.1991
Published
1991-04-00
Pages
2282-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359930
Subset
IM
Grants
NIGMS NIH HHS · GM 31577 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.4.2282-2290.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2