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PMID: 200698 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The interaction of Herpes Simplex Virus with murine lymphocytes. I. Mitogenic properties of herpes simplex virus.

The Journal of experimental medicine ·Vol. 146 ·No. 6 ·1977-12-01 ·Pages 1500-10

Mochizuki D, Hedrick S, Watson J, Kingsbury DT

Abstract

Herpes simplex virus (HSV) stimulates DNA synthesis in mouse spleen cultures prepared from normal, macrophage-depleted, and T-cell-depleted spleen cells, but not from thymocytes. In addition, a polyclonal antibody response is observed in HSV-infected spleen cultures. These findings indicate that the cells stimulated to undergo DNA synthesis after HSV infection appear to be the bone marrow-derived lymphocytes. The newly synthesized DNA is host cell and not of viral origin. Heat treatment and ultraviolet irradiation of HSV before addition to spleen cultures prevents the induction of DNA synthesis. We consider the use of this system as assay for the study of cell transformation by HSV and also for the study of host cell control of the expression of the viral genome.

MeSH Terms
Animals Cell Transformation, Viral DNA, Viral/biosynthesis Lymphocytes Mice Mitogens Simplexvirus
Chemicals
DNA, Viral Mitogens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mochizuki D
Hedrick S
Watson J
Kingsbury D T
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22 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.146.6.1500
Published
1977-12-01
Pages
1500-10
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2181902
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.146.6.1500 PMC Full Text View journal details
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