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PMID: 2007633 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria.

Journal of clinical microbiology ·Vol. 29 ·No. 2 ·1991-02-00 ·Pages 264-71

Wasem CF, McCarthy CM, Murray LW

Abstract

Multilocus enzyme electrophoresis analysis was used to evaluate the Mycobacterium avium complex (MAC), M. paratuberculosis, and nine other mycobacterial species. The average number of alleles per locus was 2.8 for the 35 MAC and 2 M. paratuberculosis strains which represented 24 electrophoretic types (ETs) and two distinct groups. The M. avium group was resolved into 17 ETs and contained the M. paratuberculosis ET. The M. intracellulare group consisted of six ETs. There was complete agreement between Gen-Probe identification and group placement by multilocus enzyme electrophoresis. The mean genetic diversity per locus for the 24 MAC ETs was 0.38. This procedure subdivided some serovars and, if implemented, should prove to be a powerful epidemiologic tool for the MAC. Eleven additional ETs were formed after the data for the other mycobacterial species were pooled with those for the MAC.

MeSH Terms
Alleles Electrophoresis, Starch Gel Enzymes/genetics,isolation & purification Genetic Variation Humans Mycobacterium/enzymology,genetics,isolation & purification Mycobacterium Infections/diagnosis Mycobacterium avium Complex/enzymology,genetics,isolation & purification Mycobacterium avium-intracellulare Infection/diagnosis Species Specificity
Chemicals
Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wasem C F
Department of Biology, New Mexico State University, Las Cruces 88003-0001.
McCarthy C M
Murray L W
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.29.2.264-271.1991
Published
1991-02-00
Pages
264-71
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC269751
Subset
IM
Grants
NCRR NIH HHS · S07 RR07154 · United States
External Links
PubMed source DOI: 10.1128/jcm.29.2.264-271.1991 PMC Full Text View journal details
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