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PMID: 20081834 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

FRT-seq: amplification-free, strand-specific transcriptome sequencing.

Nature methods ·Vol. 7 ·No. 2 ·2010-02-00 ·Pages 130-2

Mamanova L, Andrews RM, James KD, Sheridan EM, Ellis PD, Langford CF, Ost TW, Collins JE, Turner DJ

Abstract

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.

MeSH Terms
Chromosome Mapping/methods Fluorescence Resonance Energy Transfer/methods Gene Expression Profiling/methods Nucleic Acid Amplification Techniques Sequence Analysis, DNA/methods Transcription Factors/genetics
Chemicals
Transcription Factors
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Mamanova Lira
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
Andrews Robert M
James Keith D
Sheridan Elizabeth M
Ellis Peter D
Langford Cordelia F
Ost Tobias W B
Collins John E
Turner Daniel J
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.1417
Published
2010-02-00
Epub
2010-00-17
Pages
130-2
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2861772
Subset
IM
Grants
Wellcome Trust · 079643 · United Kingdom
External Links
PubMed source DOI: 10.1038/nmeth.1417 PMC Full Text View journal details
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