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PMID: 20097791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cross-kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses.

Plant physiology ·Vol. 152 ·No. 3 ·2010-03-00 ·Pages 1484-500

Mustroph A, Lee SC, Oosumi T, Zanetti ME, Yang H, Ma K, Yaghoubi-Masihi A, Fukao T, Bailey-Serres J

Abstract

High-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, or complete submergence. We showed previously that Arabidopsis (Arabidopsis thaliana) seedlings elevate the levels of hundreds of transcripts, including a core group of 49 genes that are prioritized for translation across cell types of both shoots and roots. To recognize low-oxygen responses that are evolutionarily conserved versus species specific, we compared the transcriptomic reconfiguration in 21 organisms from four kingdoms (Plantae, Animalia, Fungi, and Bacteria). Sorting of organism proteomes into clusters of putative orthologs identified broadly conserved responses associated with glycolysis, fermentation, alternative respiration, metabolite transport, reactive oxygen species amelioration, chaperone activity, and ribosome biogenesis. Differentially regulated genes involved in signaling and transcriptional regulation were poorly conserved across kingdoms. Strikingly, nearly half of the induced mRNAs of Arabidopsis seedlings encode proteins of unknown function, of which over 40% had up-regulated orthologs in poplar (Populus trichocarpa), rice (Oryza sativa), or Chlamydomonas reinhardtii. Sixteen HYPOXIA-RESPONSIVE UNKNOWN PROTEIN (HUP) genes, including four that are Arabidopsis specific, were ectopically overexpressed and evaluated for their effect on seedling tolerance to oxygen deprivation. This allowed the identification of HUPs coregulated with genes associated with anaerobic metabolism and other processes that significantly enhance or reduce stress survival when ectopically overexpressed. These findings illuminate both broadly conserved and plant-specific low-oxygen stress responses and confirm that plant-specific HUPs with limited phylogenetic distribution influence low-oxygen stress endurance.

MeSH Terms
Arabidopsis/genetics,metabolism Chlamydomonas/genetics,metabolism Cluster Analysis Comparative Genomic Hybridization Computational Biology Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Hypoxia Oryza/genetics,metabolism Oxygen/metabolism Plants, Genetically Modified/genetics,metabolism Populus/genetics,metabolism Species Specificity Stress, Physiological
Chemicals
Oxygen
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Mustroph Angelika
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, California 92521, USA.
Lee Seung Cho
Oosumi Teruko
Zanetti Maria Eugenia
Yang Huijun
Ma Kelvin
Yaghoubi-Masihi Arbi
Fukao Takeshi
Bailey-Serres Julia
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.109.151845
Published
2010-03-00
Epub
2010-00-22
Pages
1484-500
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2832244
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.109.151845 PMC Full Text View journal details
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