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PMID: 2011739 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.

Science (New York, N.Y.) ·Vol. 251 ·No. 5001 ·1991-03-29 ·Pages 1597-600

Gatignol A, Buckler-White A, Berkhout B, Jeang KT

Abstract

Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.

MeSH Terms

Amino Acid Sequence Base Sequence Binding Sites Carrier Proteins/genetics Endoribonucleases/genetics Escherichia coli/enzymology Escherichia coli Proteins Gene Products, tat/metabolism HIV Long Terminal Repeat HIV-1/genetics Humans Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Viral/genetics RNA-Binding Proteins Ribonuclease III Sequence Homology, Nucleic Acid tat Gene Products, Human Immunodeficiency Virus

Chemicals

Carrier Proteins Escherichia coli Proteins Gene Products, tat RNA, Viral RNA-Binding Proteins tat Gene Products, Human Immunodeficiency Virus trans-activation responsive RNA-binding protein Endoribonucleases Ribonuclease III ribonuclease III, E coli

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gatignol A

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Buckler-White A

Berkhout B

Jeang K T

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.2011739

Published

1991-03-29

Pages

1597-600

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

M55283, M60801, M60861, S71725, X53015, X53016, X53017, X53018, X53019, X53020, X53021

External Links

PubMed source DOI: 10.1126/science.2011739 View journal details

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Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.

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