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PMID: 20130169 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 30 ·No. 5 ·2010-02-03 ·Pages 1582-94

Miyoshi G, Hjerling-Leffler J, Karayannis T, Sousa VH, Butt SJ, Battiste J, Johnson JE, Machold RP, Fishell G

Abstract

By combining an inducible genetic fate mapping strategy with electrophysiological analysis, we have systematically characterized the populations of cortical GABAergic interneurons that originate from the caudal ganglionic eminence (CGE). Interestingly, compared with medial ganglionic eminence (MGE)-derived cortical interneuron populations, the initiation [embryonic day 12.5 (E12.5)] and peak production (E16.5) of interneurons from this embryonic structure occurs 3 d later in development. Moreover, unlike either pyramidal cells or MGE-derived cortical interneurons, CGE-derived interneurons do not integrate into the cortex in an inside-out manner but preferentially (75%) occupy superficial cortical layers independent of birthdate. In contrast to previous estimates, CGE-derived interneurons are both considerably greater in number (approximately 30% of all cortical interneurons) and diversity (comprised by at least nine distinct subtypes). Furthermore, we found that a large proportion of CGE-derived interneurons, including the neurogliaform subtype, express the glycoprotein Reelin. In fact, most CGE-derived cortical interneurons express either Reelin or vasoactive intestinal polypeptide. Thus, in conjunction with previous studies, we have now determined the spatial and temporal origins of the vast majority of cortical interneuron subtypes.

MeSH Terms
Animals Body Patterning/genetics Cell Adhesion Molecules, Neuronal/metabolism Cell Differentiation/genetics Cell Lineage/genetics Cerebral Cortex/cytology,embryology,metabolism,physiology Extracellular Matrix Proteins/metabolism Genetic Markers Genetic Techniques Interneurons/cytology,metabolism,physiology Male Mice Nerve Tissue Proteins/metabolism Patch-Clamp Techniques Prosencephalon/cytology,physiology Reelin Protein Serine Endopeptidases/metabolism
Chemicals
Cell Adhesion Molecules, Neuronal Extracellular Matrix Proteins Genetic Markers Nerve Tissue Proteins Reelin Protein Reln protein, mouse Serine Endopeptidases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Miyoshi Goichi
Smilow Neuroscience Program and the Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA.
Hjerling-Leffler Jens
Karayannis Theofanis
Sousa Vitor H
Butt Simon J B
Battiste James
Johnson Jane E
Machold Robert P
Fishell Gord
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.4515-09.2010
Published
2010-02-03
Pages
1582-94
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC2826846
Subset
IM
Grants
NINDS NIH HHS · R01NS039007 · United States
NIMH NIH HHS · R01 MH071679 · United States
NIMH NIH HHS · R01MH071679 · United States
NIMH NIH HHS · R01 MH071679-03 · United States
NINDS NIH HHS · R01 NS039007 · United States
NIMH NIH HHS · R01 MH071679-02 · United States
NIMH NIH HHS · R01 MH071679-01A1 · United States
NIMH NIH HHS · R01 MH071679-04 · United States
NINDS NIH HHS · R01 NS039007-08 · United States
NINDS NIH HHS · R01 NS039007-09A1 · United States
NIMH NIH HHS · R01 MH071679-05 · United States
NINDS NIH HHS · R01 NS039007-10 · United States
Corrections
CommentIn
PMID: 20519518
External Links
PubMed source DOI: 10.1523/JNEUROSCI.4515-09.2010 PMC Full Text View journal details
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