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PMID: 2016777 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High-efficiency formation of influenza virus transfectants.

Journal of virology ·Vol. 65 ·No. 5 ·1991-05-00 ·Pages 2711-3

Enami M, Palese P

Abstract

cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.

Related Genes
HA NS
MeSH Terms
Animals Base Sequence Cattle Cell Line DNA, Viral Electrophoresis, Polyacrylamide Gel Genes, Viral Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/genetics,metabolism Molecular Sequence Data Neuraminidase/genetics,metabolism Orthomyxoviridae/genetics RNA, Viral/genetics Ribonucleoproteins/metabolism Transfection
Chemicals
DNA, Viral Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral RNA, Viral Ribonucleoproteins Neuraminidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Enami M
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029.
Palese P
References (9)
9 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.5.2711-2713.1991
Published
1991-05-00
Pages
2711-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240634
Subset
IM
Grants
PHS HHS · A124460 · United States
NIAID NIH HHS · AI11823 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.5.2711-2713.1991 PMC Full Text View journal details
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